Li Huai-Bin, Wu Feng, Miao Hua-Chun, Xiong Ke-Ren
Department of Anatomy, Wannan Medical College, Wuhu, Anhui, China (mainland).
Med Sci Monit Basic Res. 2016 Dec 23;22:175-180. doi: 10.12659/MSMBR.901524.
BACKGROUND The aim of this study was to explore the neural protective effect of polysaccharide of Gastrodia elata Blume (PGB) and electro-acupuncture (EA) on focal cerebral ischemia rats. MATERIAL AND METHODS A total of 40 Sprague-Dawley rats were randomly divided into 5 groups (normal group, model group, PGB group, EA group and PGB+EA group). The model was prepared by middle cerebral artery occlusion (MCAO). Two week after modeling, rats were given PGB, EA, or a combination of the 2 in continuous treatment for 2 successive weeks. 14 days after modeling, expressions of BDNF and SCF protein in the caudate putamen (CPu) were detected by immunohistochemistry. RESULTS Positive expression of BDNF and SCF protein was found in the right caudate putamen of each group of rats. Expressions of BDNF and SCF in the CPu of the model group were higher than normal group (P<0.05). Compared with the model group, the expressions of BDNF and SCF in the CPu of the PGB group, the EA group, and the PGB plus EA group increased significantly (P<0.05). The expressions of BDNF and SCF obviously increased in the PGB plus EA group compared to those of the EA group and the PGB group (P<0.05). CONCLUSIONS PGB and EA up-regulated the expressions of BDNF and SCF protein in the CPu of focal cerebral ischemia rats, and the combination of PGB+EA has a synergistic effect on the recovery from cerebral ischemia.
背景 本研究旨在探讨天麻多糖（PGB）和电针（EA）对局灶性脑缺血大鼠的神经保护作用。
材料与方法 将40只Sprague-Dawley大鼠随机分为5组（正常组、模型组、PGB组、EA组和PGB+EA组）。采用大脑中动脉闭塞（MCAO）法制备模型。造模2周后，大鼠连续2周接受PGB、EA或二者联合治疗。造模14天后，采用免疫组织化学法检测尾状核（CPu）中脑源性神经营养因子（BDNF）和干细胞因子（SCF）蛋白的表达。
结果 各组大鼠右侧尾状核均发现BDNF和SCF蛋白的阳性表达。模型组CPu中BDNF和SCF的表达高于正常组（P<0.05）。与模型组相比，PGB组、EA组和PGB+EA组CPu中BDNF和SCF的表达显著增加（P<0.05）。与EA组和PGB组相比，PGB+EA组BDNF和SCF的表达明显增加（P<0.05）。
结论 PGB和EA上调局灶性脑缺血大鼠CPu中BDNF和SCF蛋白的表达，PGB+EA联合应用对脑缺血恢复具有协同作用。
