采用不对称流场流分离技术评估多糖结构修饰对抗乳腺癌活性的影响。
Assessment of the Effects of Structural Modification of Polysaccharide on Anti-Breast Cancer Activity Using Asymmetrical Flow Field-Flow Fractionation.
机构信息
Key Laboratory of Pathogenesis Mechanism and Control of Inflammatory-Autoimmune Disease of Hebei Province, School of Basic Medical Sciences, Hebei University, Baoding 071000, China.
Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, College of Chemistry and Materials and Science, Hebei University, Baoding 071002, China.
出版信息
Molecules. 2023 Jun 9;28(12):4669. doi: 10.3390/molecules28124669.
("Tian Ma" in Chinese) is used as a food and medical ingredient in traditional Chinese medicine. In this study, to enhance the anti-breast cancer activity of polysaccharide (GEP), GEPs were modified via sulfidation (SGEP) and acetylation (AcGEP). The physicochemical properties (such as solubility and substitution degree) and structural information (such as molecular weight and radius of gyration ) of GEP derivatives were determined by Fourier transformed infrared (FTIR) spectroscopy and asymmetrical flow field-flow fractionation (AF4) coupled online with multiangle light scattering (MALS) and differential refractive index (dRI) detectors (AF4-MALS-dRI). The effects of the structural modification of GEP on the proliferation, apoptosis, and cell cycle of MCF-7 cell were studied systematically. The ability of MCF-7 cell for the uptake of GEP was studied by laser scanning confocal microscopy (LSCM). The results suggested that the solubility and anti-breast cancer activity of GEP were enhanced and the average and of GEP decreased after chemical modification. The AF4-MALS-dRI results showed that the chemical modification process simultaneously caused the degradation and aggregation of GEPs. The LSCM results revealed that more SGEP can enter the MCF-7 cell interior compared with AcGEP. The results indicated that the structure of AcGEP could play a dominating role in antitumor activity. The data obtained in this work can be used as a starting point for investigating the structure-bioactivity of GEPs.
（中文名称：天麻）在中国传统医学中既被用作食品，也被用作药材。在这项研究中，为了增强多糖（GEP）的抗乳腺癌活性，通过磺化（SGEP）和乙酰化（AcGEP）对 GEP 进行了修饰。采用傅里叶变换红外（FTIR）光谱法和不对称流场流分离（AF4）与多角度光散射（MALS）和差示折射指数（dRI）检测器在线联用（AF4-MALS-dRI），测定了 GEP 衍生物的物理化学性质（如溶解度和取代度）和结构信息（如分子量和回转半径）。系统研究了 GEP 结构修饰对 MCF-7 细胞增殖、凋亡和细胞周期的影响。采用激光扫描共聚焦显微镜（LSCM）研究了 MCF-7 细胞对 GEP 的摄取能力。结果表明，GEP 的溶解度和抗乳腺癌活性得到增强，且化学修饰后 GEP 的 和 降低。AF4-MALS-dRI 结果表明，化学修饰过程同时导致了 GEP 的降解和聚集。LSCM 结果表明，与 AcGEP 相比，更多的 SGEP 可以进入 MCF-7 细胞内部。结果表明，AcGEP 的结构在抗肿瘤活性中起主导作用。本工作获得的数据可作为研究 GEP 结构-生物活性的起点。
Zotero 插件