Department of Chemistry, Faculty of Science, Vali-e-Asr University of Rafsanjan, 77188-97111, Iran; High Temperature Fuel Cell Research Group, Vali-e-Asr University of Rafsanjan, 77188-97111, Iran.
Department of Chemistry, Faculty of Science, Vali-e-Asr University of Rafsanjan, 77188-97111, Iran.
Biosens Bioelectron. 2017 May 15;91:284-292. doi: 10.1016/j.bios.2016.12.048. Epub 2016 Dec 22.
A densely packed gold nanoparticles on the rGO-MWCNT platform was used as the basis for an ultrasensitive label-free electrochemical aptasensor to detect the biomarker prostate specific antigen (PSA) in serum. The detection was based on that the variation of electron transfer resistance (R) and differential pulse voltammetry (DPV) current were relevant to the formation of PSA-aptamer complex at the modified electrode surface. Compared with pure AuNPs, rGO-MWCNT and MWCNT/AuNPs, the rGO-MWCNT/AuNPs nanocomposite modified electrode was the most sensitive aptasensing platform for the determination of PSA. Two calibration curves were prepared from the data obtained from the DPV and electrochemical impedance spectroscopy (EIS) by plotting the peak current and R against PSA concentration, respectively. The proposed aptasensor had an extremely low LOD of 1.0pgmL PSA within the detection range of 0.005-20ngmL and 0.005-100ngmL for DPV and EIS calibration curves, respectively. This sensor exhibited outstanding anti-interference ability towards co-existing molecules with good stability, sensitivity, and reproducibility. Clinical application was performed with analysis of the PSA levels in serum samples obtained from patients with prostate cancer using both the aptasensor and Immunoradiometric assay. The results revealed the proposed system to be a promising candidate for clinical analysis of PSA.
在 rGO-MWCNT 平台上密集排列的金纳米粒子被用作一种超灵敏的无标记电化学适体传感器的基础，用于检测血清中的生物标志物前列腺特异性抗原（PSA）。该检测是基于在修饰电极表面形成 PSA-适体复合物时，电子转移电阻（R）和差分脉冲伏安法（DPV）电流的变化。与纯 AuNPs、rGO-MWCNT 和 MWCNT/AuNPs 相比，rGO-MWCNT/AuNPs 纳米复合材料修饰电极是用于测定 PSA 的最灵敏的适体传感平台。通过绘制 DPV 和电化学阻抗谱（EIS）获得的数据的峰值电流和 R 与 PSA 浓度的关系，分别从 DPV 和 EIS 校准曲线中获得两个校准曲线。所提出的适体传感器具有极低的 LOD，对于 DPV 和 EIS 校准曲线，其检测范围分别为 0.005-20ngmL 和 0.005-100ngmL 时，PSA 的 LOD 分别为 1.0pgmL。该传感器对共存分子具有出色的抗干扰能力，稳定性、灵敏度和重现性良好。使用适体传感器和免疫放射分析对来自前列腺癌患者的血清样本中的 PSA 水平进行了临床应用。结果表明，该系统是用于 PSA 临床分析的有前途的候选者。
