巴基斯坦无法分型的丙型肝炎病毒亚型：一个被忽视的领域。

Untypeable hepatitis C virus subtypes in Pakistan: A neglected section.

作者信息

Khan Abdul Waheed, Nasim Zeeshan, Zahir Fazli, Ali Shahid, Ali Abid, Iqbal Aqib, Munir Iqbal

机构信息

1 Institute of Biotechnology and Genetic Engineering, The University of Agriculture , Peshawar, Pakistan.

2 Center for Biotechnology and Microbiology, University of Swat , Odigram, Pakistan.

出版信息

Acta Microbiol Immunol Hung. 2016 Dec;63(4):427-431. doi: 10.1556/030.63.2016.025.

DOI:10.1556/030.63.2016.025

PMID:28033725

Abstract

Diagnostically untypeable subtypes contribute a considerable percent of hepatitis C virus (HCV) subtypes in Pakistan. In the present study, chronically infected HCV patients with known viremia were subjected to HCV genotyping. Among the total retrieved samples, 92.7% (64/69) were found typeable while 7.24% (5/69) were diagnostically untypeable. In conclusion, the presence of large number of untypeable HCV subtypes emphasizes the need of an updated type-specific genotyping assay and consideration of primers for proportionally rare subtypes to minimize the number of untypeable HCV subtypes.

摘要

在巴基斯坦，无法进行诊断分型的丙型肝炎病毒（HCV）亚型占相当比例。在本研究中，对已知病毒血症的慢性HCV感染患者进行了HCV基因分型。在总共检索到的样本中，92.7%（64/69）可分型，而7.24%（5/69）无法进行诊断分型。总之，大量无法分型的HCV亚型的存在强调了需要一种更新的型特异性基因分型检测方法，并考虑针对比例罕见亚型的引物，以尽量减少无法分型的HCV亚型数量。