Yoshioka H, Iino S, Sato N, Osamura T, Hasegawa K, Ochi M, Sawada T, Kusunoki T
Department of Pediatric, Kyoto Prefectural University of Medicine, Japan.
Pediatr Neurol. 1989 Jul-Aug;5(4):221-5. doi: 10.1016/0887-8994(89)90079-9.
An easy and inexpensive method is reported for producing hemorrhagic brain damage in newborn mice, involving only exposure to hypoxia. One-day-old mice, Jcl:ICR strain, were subjected to a humidified 5% oxygen, 95% nitrogen mixture for 8 hours. After the hypoxic episode, 34% of newborn mice survived, 59% manifested cerebral parenchymal hemorrhage. Cortical hemorrhage could be detected in live mice; intracranial hemorrhage was observed through the thin skin and skull. Cortical hemorrhage usually affected the bilateral parietal regions symmetrically and neuronal destruction was observed in the deeper structures, as well as in the cerebral cortex. This pattern of damage was comparable to parasagittal cerebral injury in humans. The onset of cortical hemorrhage and neuropathology in these mice suggested that hemorrhage occurred when cerebral blood flow recovered after the hypoxic event.
据报道，有一种简单且成本低廉的方法可在新生小鼠中造成出血性脑损伤，仅需使其暴露于低氧环境。选用1日龄的Jcl:ICR品系小鼠，使其暴露于含5%氧气和95%氮气的湿润混合气体中8小时。缺氧发作后，34%的新生小鼠存活，59%表现出脑实质出血。在活体小鼠中可检测到皮质出血；透过薄皮肤和颅骨可观察到颅内出血。皮质出血通常对称地累及双侧顶叶区域，在更深层结构以及大脑皮质中均观察到神经元破坏。这种损伤模式与人类矢状旁脑损伤相似。这些小鼠中皮质出血和神经病理学的发作表明，出血发生在缺氧事件后脑血流恢复时。
