Chauhan Neetu, Dilbaghi Neeraj, Gopal Madhuban, Kumar Rajesh, Kim Ki-Hyun, Kumar Sandeep
Department of Bio and Nano Technology, Guru Jambheshwar University of Science & Technology, Hisar 125001, India.
Division of Agricultural Chemicals, Indian Agricultural Research Institute (IARI), New Delhi 110012, India.
Int J Biol Macromol. 2017 Apr;97:616-624. doi: 10.1016/j.ijbiomac.2016.12.059. Epub 2016 Dec 26.
Accelerated use of pesticides in cutting edge agriculture prompted us to explore smart nanoformulations to subside the consumption of these perilous chemicals. Polymer nanocapsules carrying a fungicide, hexaconazole were developed through ionotropic gelation method utilizing chitosan and tripolyphosphate (TPP). The nanocapsules were characterized by photon correlation spectroscope (PCS), transmission electron microscope (TEM), and Fourier transform infra-red (FTIR) spectroscope. Nanocapsules were optimized for size and high encapsulation efficiency using central composite design (CCD) software. The encapsulation efficiency of nanocapsules for hexaconazole was 73% as assessed by gas chromatography (GC). Nanocapsules were analysed and compared with commercial formulation for controlled release in vitro at three different pH values. Release of hexaconazole from nanocapsules was fastest at pH 4 in comparison to pH 7 and pH 10. Release study in soil was also conducted and revealed a controlled pattern for nanoformulation. The fungicidal activity of the prepared nanoformulation was evaluated against R. solani and was compared with commercial formulation of hexaconazole. The cytotoxicity assay performed on vero cell lines by MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) assay confirmed that nanoformulation is less toxic than commercial formulation of pesticide.
前沿农业中农药的加速使用促使我们探索智能纳米制剂，以减少这些危险化学品的使用量。通过离子凝胶法，利用壳聚糖和三聚磷酸钠（TPP）制备了载有杀菌剂己唑醇的聚合物纳米胶囊。通过光子相关光谱仪（PCS）、透射电子显微镜（TEM）和傅里叶变换红外（FTIR）光谱仪对纳米胶囊进行了表征。使用中心复合设计（CCD）软件对纳米胶囊的尺寸和高包封率进行了优化。通过气相色谱（GC）评估，纳米胶囊对己唑醇的包封率为73%。分析了纳米胶囊，并与市售制剂在三种不同pH值下的体外控释情况进行了比较。与pH 7和pH 10相比，己唑醇在pH 4时从纳米胶囊中的释放速度最快。还进行了土壤中的释放研究，结果表明纳米制剂具有可控的释放模式。评估了所制备的纳米制剂对茄丝核菌的杀菌活性，并与己唑醇的市售制剂进行了比较。通过MTT（3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐）法对 vero 细胞系进行的细胞毒性试验证实，纳米制剂的毒性低于农药市售制剂。
