Park Sang A, Hyun Young-Min
Department of Anatomy, Yonsei University College of Medicine, Seoul 03722, Korea.
Immune Netw. 2016 Dec;16(6):317-321. doi: 10.4110/in.2016.16.6.317. Epub 2016 Dec 22.
Immune cells (leukocytes or white blood cells) move actively and sensitively based on body conditions. Despite their important role as protectors inside the body, it is difficult to directly observe the spatiotemporal momentum of leukocytes. With advances in imaging technology, the introduction of two-photon microscopy has enabled researchers to look deeper inside tissues in a three-dimensional manner. In observations of immune cell movement along the blood vessel, vascular permeability and innate immune cell movements remain unclear. Here, we describe the neutrophil extravasation cascade, which were observed using a two-photon intravital imaging technique. We also provide evidence for novel mechanisms such as neutrophil body extension and microparticle formation as well as their biological roles during migration.
免疫细胞（白细胞或白血球）会根据身体状况进行积极且灵敏的移动。尽管它们作为体内的保护者发挥着重要作用，但直接观察白细胞的时空动态却很困难。随着成像技术的进步，双光子显微镜的引入使研究人员能够以三维方式更深入地观察组织内部。在观察免疫细胞沿血管的移动时，血管通透性和先天免疫细胞的移动仍不清楚。在此，我们描述了使用双光子活体成像技术观察到的中性粒细胞渗出级联反应。我们还为中性粒细胞体延伸和微粒形成等新机制及其在迁移过程中的生物学作用提供了证据。
