Department of Mechanical Engineering, Khalifa University, Abu Dhabi, UAE.
Current address: Department of Mechanical Engineering, United Arab Emirates University, Al Ain, UAE.
J Sep Sci. 2017 Mar;40(5):1193-1200. doi: 10.1002/jssc.201601061. Epub 2017 Jan 30.
We describe the design, microfabrication, and testing of a microfluidic device for the separation of cancer cells based on dielectrophoresis. Cancer cells, specifically green fluorescent protein-labeled MDA-MB-231, are successfully separated from a heterogeneous mixture of the same and normal blood cells. MDA-MB-231 cancer cells are separated with an accuracy that enables precise detection and counting of circulating tumor cells present among normal blood cells. The separation is performed using a set of planar interdigitated transducer electrodes that are deposited on the surface of a glass wafer and slightly protrude into the separation microchannel at one side. The device includes two parts, namely, a glass wafer and polydimethylsiloxane element. The device is fabricated using standard microfabrication techniques. All experiments are conducted with low conductivity sucrose-dextrose isotonic medium. The variation in response between MDA-MB-231 cancer cells and normal cells to a certain band of alternating-current frequencies is used for continuous separation of cells. The fabrication of the microfluidic device, preparation of cells and medium, and flow conditions are detailed. The proposed microdevice can be used to detect and separate malignant cells from heterogeneous mixture of cells for the purpose of early screening for cancer.
我们描述了一种基于介电泳的用于分离癌细胞的微流控装置的设计、微制造和测试。成功地从相同的和正常的血细胞的异质混合物中分离出癌细胞，特别是绿色荧光蛋白标记的 MDA-MB-231。从正常血细胞中存在的循环肿瘤细胞中进行精确检测和计数，以实现 MDA-MB-231 癌细胞的精确分离。分离是通过在玻璃晶片表面沉积的一组平面叉指换能器电极来实现的，这些电极在一侧稍微突出到分离微通道中。该装置包括两个部分，即玻璃晶片和聚二甲基硅氧烷元件。该装置采用标准的微制造技术制造。所有实验均在低电导率蔗糖-葡萄糖等渗介质中进行。使用 MDA-MB-231 癌细胞和正常细胞对一定频率的交流电流的响应变化来进行连续的细胞分离。详细介绍了微流控装置的制造、细胞和介质的制备以及流动条件。所提出的微器件可用于从细胞的异质混合物中检测和分离恶性细胞，以便早期筛查癌症。
