Mechanical Engineering Department, United Arab Emirates University, Al Ain, United Arab Emirates; National Water Center, United Arab Emirates University, Al Ain, United Arab Emirates.
Mechanical Engineering Department, United Arab Emirates University, Al Ain, United Arab Emirates; National Water Center, United Arab Emirates University, Al Ain, United Arab Emirates.
Med Eng Phys. 2020 Jul;81:130-135. doi: 10.1016/j.medengphy.2020.05.017. Epub 2020 May 22.
This article details simulation based study of cell separation in a dielectrophoretic microfluidic device. The device consists of a narrow microchannel connected to a wide microchannel with several finite sized planar interdigitated transducer electrodes protruding into the narrow microchannel from one of its sidewalls. In the narrow microchannel, the circulating tumor cells are subjected to positive dielectrophoresis while the regular cells are subjected to negative dielectrophoresis to achieve separation and as all cells move in to the wide microchannel, the physical distance between the two types of cells increases thereby making their collection from the device easier. Equations describing motion, fluid field, electric field, and electric potential form the mathematical model and accounts for forces related to inertia, drag, and dielectrophoresis. Applied electric potential, electrode/gap length, and tumor cell diameter have a positive effect on the performance metrics while velocity of the medium and microchannel width have negative effect on the performance metrics. The model presented in this article is beneficial in realizing liquid biopsy with the desired performance metrics using the proposed microfluidic device.
本文详细介绍了基于仿真的介电泳微流控芯片细胞分离研究。该装置由一个窄微通道与一个宽微通道相连，在宽微通道的一侧壁内有几个有限尺寸的平面叉指式换能器电极突出进入窄微通道。在窄微通道中，循环肿瘤细胞受到正介电泳力作用，而正常细胞受到负介电泳力作用，从而实现分离；当所有细胞进入宽微通道时，两种类型细胞之间的物理距离增大，从而更容易从装置中收集。描述运动、流场、电场和电势的方程构成了数学模型，并考虑了与惯性、阻力和介电泳力相关的力。施加的电场、电极/间隙长度和肿瘤细胞直径对性能指标有积极影响，而介质速度和微通道宽度对性能指标有消极影响。本文提出的模型有助于使用所提出的微流控装置实现具有理想性能指标的液体活检。
