Ono-Moore Kikumi D, Ferguson Matthew, Blackburn Michael L, Issafras Hassan, Adams Sean H
Arkansas Children's Nutrition Center, Little Rock, AR 72202, USA.
Department of Pediatrics, University of Arkansas for Medical Sciences, Little Rock, AR 72202, USA.
Nutrients. 2016 Dec 28;9(1):16. doi: 10.3390/nu9010016.
High-fat (HF) diets typically promote diet-induced obesity (DIO) and metabolic dysfunction (i.e., insulin resistance, hypertriglyceridemia, and hepatic steatosis). Dysfunction of triacylglycerol (TAG) metabolism may contribute to the development of hepatic steatosis, via increased de novo lipogenesis or repackaging of circulating nonesterified fatty acids (NEFAs). Hepatic TAG production (HTP) rate can be assessed through injecting mice with nonionic detergents that inhibit tissue lipoprotein lipase. Potential confounding effects of detergent-based HTP tests (HTPTs) used in longitudinal studies-including the impact on food intake, energy balance, and weight gain-have not been reported. To examine this, male C57BL/6J mice were fed a 10% or 60% kcal diet. After 4 weeks, the mice underwent an HTPT via poloxamer 407 intraperitoneal injections (1000 mg/kg). Weight gain, energy intake, and postabsorptive TAG levels normalized 7-10 days post-HTPT. The post-HTPT recovery of body weight and energy intake suggest that, in metabolic phenotyping studies, any additional sample collection should occur at least 7-10 days after the HTPT to reduce confounding effects. Diet-specific effects on HTP were also observed: HF-fed mice had reduced HTP, plasma TAG, and NEFA levels compared to controls. In conclusion, the current study highlights the procedural and physiological complexities associated with studying lipid metabolism using a HTPT in the DIO mouse model.
高脂（HF）饮食通常会导致饮食诱导的肥胖（DIO）和代谢功能障碍（即胰岛素抵抗、高甘油三酯血症和肝脂肪变性）。三酰甘油（TAG）代谢功能障碍可能通过增加从头脂肪生成或循环非酯化脂肪酸（NEFA）的重新包装，促进肝脂肪变性的发展。肝TAG生成（HTP）率可以通过给小鼠注射抑制组织脂蛋白脂肪酶的非离子洗涤剂来评估。纵向研究中使用的基于洗涤剂的HTP测试（HTPTs）的潜在混杂效应——包括对食物摄入、能量平衡和体重增加的影响——尚未见报道。为了研究这一点，给雄性C57BL/6J小鼠喂食10%或60%千卡的饮食。4周后，通过腹腔注射泊洛沙姆407（1000 mg/kg）对小鼠进行HTPT。HTPT后7-10天，体重增加、能量摄入和吸收后TAG水平恢复正常。HTPT后体重和能量摄入的恢复表明，在代谢表型研究中，任何额外的样本采集应在HTPT后至少7-10天进行，以减少混杂效应。还观察到饮食对HTP的特异性影响：与对照组相比，高脂喂养的小鼠HTP、血浆TAG和NEFA水平降低。总之，当前研究强调了在DIO小鼠模型中使用HTPT研究脂质代谢相关的程序和生理复杂性。
