Winton D J, Blount M A, Ponder B A
Institute of Cancer Research, Haddow Laboratories, Sutton, Surrey, UK.
Br J Cancer. 1989 Jul;60(1):59-63. doi: 10.1038/bjc.1989.220.
We show that, from the earliest morphologically recognisable stages of development, mouse skin papillomas induced by a chemical initiation-promotion regime are polyclonal. We have demonstrated polyclonality directly by immunohistochemical staining of the mosaic cell populations in embryo aggregation chimaeras, a method which removes some of the uncertainties of previous conclusions based on analysis using electrophoretic polymorphisms. The findings may imply that initiation within a single cell and promotion of its clonal descendents is not a sufficient explanation for the origin of these tumours and that interaction between cells of more than one clone is involved.
我们发现，从最早形态可识别的发育阶段开始，由化学启动-促进机制诱导的小鼠皮肤乳头状瘤是多克隆的。我们通过对胚胎聚集嵌合体中镶嵌细胞群体进行免疫组织化学染色直接证明了多克隆性，该方法消除了以往基于电泳多态性分析得出结论的一些不确定性。这些发现可能意味着，单个细胞内的启动及其克隆后代的促进并不能充分解释这些肿瘤的起源，并且涉及一个以上克隆的细胞之间的相互作用。
