Meng X, Ma Q, Bai H, Wang Z, Han C, Wang C
Chinese Academy of Inspection and Quarantine, Beijing, 100176, China.
College of Chemistry, Jilin University, Changchun, 130012, China.
Int J Cosmet Sci. 2017 Aug;39(4):386-392. doi: 10.1111/ics.12387. Epub 2017 Feb 2.
A comprehensive methodology for the simultaneous determination of 15 multiclass organic UV filters in sunscreen cosmetics was developed using high-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS).
Sunscreen cosmetics of various matrices, such as toning lotion, emulsion, cream and lipstick, were analysed. Ultrasound-assisted extraction (UAE) was utilized as the extraction technique for sample preparation. The 15 UV filters were chromatographically separated by two groups of mobile phase system on an XBridge C analytical column (150 × 2.1 mm I.D., 3.5 μm particle size) and quantified using HPLC-ESI-MS/MS. The quantitation was performed using the external calibration method.
The established method was validated in terms of linearity, sensitivity, specificity, accuracy, stability, intraday and interday precisions, recovery and matrix effect. The method was also applied for the determination of UV filters in commercial sunscreen cosmetics.
The experimental results demonstrated that the developed method was accurate, rapid and sensitive and can be used for the analytical control of sunscreen cosmetics.
建立了一种采用高效液相色谱-电喷雾电离串联质谱法（HPLC-ESI-MS/MS）同时测定防晒化妆品中15种多类有机紫外线过滤剂的综合方法。
分析了各种基质的防晒化妆品，如爽肤水、乳液、面霜和口红。采用超声辅助萃取（UAE）作为样品制备的萃取技术。15种紫外线过滤剂在XBridge C分析柱（内径150×2.1 mm，粒径3.5μm）上通过两组流动相系统进行色谱分离，并使用HPLC-ESI-MS/MS进行定量。定量采用外标法。
该方法在线性、灵敏度、特异性、准确性、稳定性、日内和日间精密度、回收率和基质效应方面得到了验证。该方法还应用于商业防晒化妆品中紫外线过滤剂的测定。
实验结果表明，所建立的方法准确、快速、灵敏，可用于防晒化妆品的分析控制。
