Su Xiaowen, Liu Xinguang, Wang Shouyu, Li Bin, Pan Taowen, Liu Dingrui, Wang Fei, Diao Yunpeng, Li Kun
College of Pharmacy, Dalian Medical University, Dalian, PR China.
Institute/College of Integrative Medicine, Dalian Medical University, Dalian, PR China.
Burns. 2017 Jun;43(4):830-838. doi: 10.1016/j.burns.2016.10.010. Epub 2016 Dec 28.
The healing of wounds has always provided challenges for the medical community whether chronic or acute. Modern and traditional medicine has proved that herbal medicine shown superiority over chemical drugs. Herein, we report an Entada phaseoloides (L.) Merr. extract with a total tannin content of 76.18% showed wound-healing promoting effect in rat model. We found significantly accelerated wound closure already on day 7 in animals treated with total Entada phaseoloides (L.) Merr. tannins (TEPT) as compared to vaseline treated controls (p<0.05). At day 15, histologically, the wounds in animals treated with TEPT were completely closed as compared to controls. In vitro, TEPT promotes fibroblast proliferation and migration into wounds of NIH3T3 with concentration range of 9.38-37.50μg/ml. TEPT also had an inhibitory action against Staphylococcus aureus with MBC of 1.5mg/ml and the result was further proved by transmission electron microscope. Thus, TEPT could promote wound shrinkage, improve healing rate and promote healing of infectious wounds in rats. And this effect may due to antibacterial activities and NIH3T3 cell pro-proliferative effect of the tannins compounds, which indicating that TEPT can be used as efficient treatment in traumatic injury.
无论伤口是慢性还是急性的，其愈合一直是医学界面临的挑战。现代医学和传统医学都已证明，草药比化学药物更具优势。在此，我们报告一种总单宁含量为76.18%的榼藤提取物在大鼠模型中显示出促进伤口愈合的作用。我们发现，与凡士林处理的对照组相比，用榼藤总单宁（TEPT）处理的动物在第7天伤口闭合就已显著加速（p<0.05）。在第15天，组织学检查显示，与对照组相比，用TEPT处理的动物伤口已完全闭合。在体外，TEPT在9.38 - 37.50μg/ml的浓度范围内可促进成纤维细胞增殖并迁移至NIH3T3细胞的伤口处。TEPT对金黄色葡萄球菌也有抑制作用，其最低抑菌浓度为1.5mg/ml，透射电子显微镜进一步证实了这一结果。因此，TEPT可促进大鼠伤口收缩，提高愈合率并促进感染伤口的愈合。这种作用可能归因于单宁化合物的抗菌活性和对NIH3T3细胞的促增殖作用，这表明TEPT可作为创伤损伤的有效治疗方法。
