Burlage Robert S, Tillmann Joshua
Department of Pharmaceutical and Administrative Science, Concordia University School of Pharmacy, 12800 N. Lake Shore Dr., Mequon, WI 53097, United States.
Department of Pharmaceutical and Administrative Science, Concordia University School of Pharmacy, 12800 N. Lake Shore Dr., Mequon, WI 53097, United States.
J Microbiol Methods. 2017 Jul;138:2-11. doi: 10.1016/j.mimet.2016.12.023. Epub 2016 Dec 28.
Biosensors are devices which utilize both an electrical component (transducer) and a biological component to study an environment. They are typically used to examine biological structures, organisms and processes. The field of biosensors has now become so large and varied that the technology can often seem impenetrable. Yet the principles which underlie the technology are uncomplicated, even if the details of the mechanisms are elusive. In this review we confine our analysis to relatively current advancements in biosensors for the detection of whole bacterial cells. This includes biosensors which rely on an added labeled component and biosensors which do not have a labeled component and instead detect the binding event or bound structure on the transducer. Methods to concentrate the bacteria prior to biosensor analysis are also described. The variety of biosensor types and their actual and potential uses are described.
生物传感器是利用电子元件(换能器)和生物元件来研究环境的设备。它们通常用于检测生物结构、生物体和生物过程。如今,生物传感器领域已经变得如此庞大和多样,以至于这项技术常常让人觉得难以理解。然而,尽管该技术机制的细节难以捉摸,但其背后的原理并不复杂。在本综述中,我们将分析局限于用于检测完整细菌细胞的生物传感器的相对最新进展。这包括依赖添加标记成分的生物传感器以及没有标记成分而是检测换能器上的结合事件或结合结构的生物传感器。还描述了在生物传感器分析之前浓缩细菌的方法。文中介绍了各种生物传感器类型及其实际和潜在用途。