Jafari Mohammad, Dadras Farahnaz, Ghadimipour Hamid Reza, Seif Rabiei Mohamad Ali, Khoshjou Farhad
Pathology ward, Hamadan University of Medical Sciences, Hamadan, Iran.
Nephrology Ward, Department of Internal Medicine, Iran University of medical Sciences, Tehran, Iran.
J Nephropathol. 2017 Jan;6(1):1-4. doi: 10.15171/jnp.2017.01. Epub 2016 Aug 3.
One of common mechanisms in pathophysiology of chronic kidney diseases is epithelial-mesenchymal transition (EMT). On the other hand oxidative stress has been known to participate in kidney damage of diabetic nephropathy (DN).
We studied if tempol, a well-known antioxidant agent, can ameliorate EMT in DN induced in male rats.
Twenty-seven male rats were equally divided in to 4 groups. Group I (control or C), group II (diabetic or D), group III (T) rats which was given tempol (100 mg/kg/day) by gavages for 28 days and group IV (D&T) was diabetic rats that also received same dose of tempol. After treatment, their kidneys were studied by immunohistochemicalstaining.
Pathological changes in the kidney were detected concurrently with increasing kidney weight and urinary albumin excretion. In addition, EMT indices, i.e. decline of E-cadherin and increase of α SMA staining were significantly emerged in the renal tubular cells of diabetic group and were partially modified in diabetic group which were simultaneously treated by tempol.
Tempol can modify, but not significantly, EMT induced by DN.
慢性肾脏病病理生理学的常见机制之一是上皮-间质转化（EMT）。另一方面，氧化应激已知参与糖尿病肾病（DN）的肾脏损伤。
我们研究了知名抗氧化剂tempol是否能改善雄性大鼠诱导的DN中的EMT。
27只雄性大鼠平均分为4组。第一组（对照组或C组），第二组（糖尿病组或D组），第三组（T组）大鼠通过灌胃给予tempol（100mg/kg/天）28天，第四组（D&T组）是同样接受相同剂量tempol的糖尿病大鼠。治疗后，通过免疫组织化学染色研究它们的肾脏。
肾脏的病理变化与肾脏重量增加和尿白蛋白排泄增加同时被检测到。此外，EMT指标，即E-钙黏蛋白下降和α-SMA染色增加，在糖尿病组肾小管细胞中显著出现，并在同时接受tempol治疗的糖尿病组中部分得到改善。
Tempol可以改变，但不显著改变DN诱导的EMT。
