Goodarzi Zahra, Karami Esmaeil, Ahmadizadeh Massumeh
Department of Occupational Health, Engineering, School of Health, Semnan University of Medical Sciences, Semnan, Iran.
Department of Occupational Health, Engineering, School of Health, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran ; Physiology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
J Nephropathol. 2017 Jan;6(1):5-9. doi: 10.15171/jnp.2017.02. Epub 2016 Aug 9.
Hexavalent Chromium (Cr (VI)) compounds are extremely toxic and have been demonstrated to induce nephrotoxicity associated with oxidative stress in humans and animals. The wide environmental distribution of these agents lead to an increase interest of preventive effects of its adverse effects.
The propose of the present study was to determine the potential protective effects of simvastatin (SIMV) on Cr (VI)-induced nephrotoxicity in rat.
Forty-eight adult male Wistar rats (180-220 g BW) were randomly assigned to eight groups (n = 6). Group one received SIMV 20 mg/kg/day. Group two was given vehicle only. Groups three, five and seven received intraperitoneally (i.p) sodium dichromate (Cr (VI)) at doses of 8, 12 and 16 mg/kg body weight. Groups four, six and eight pretreated with the 20 mg/kg SIMV 30 minutes to prior administration of Cr (VI) at doses of 8, 12 and 16 mg/kg, respectively. The experiment repeated for eight consecutive days. Twenty-four hours after the last administration, animals were killed with overdose of sodium pentobarbital. Kidney tissues were excised for measuring malondialdehyde (MDA), glutathione (GSH) and histopathological examination.
Chromium induced a dose dependent elevation of MDA and reduction of GSH levels. Histopathological manifestations were observed in Cr (VI)-treated rats. SIMV administration restored Cr (VI) produced biochemical and morphological changes in rat kidney. SIMV decreased MDA values and increased GSH levels in Cr (VI)-treated rats. SIMV clearly reversed the microscopic damage, demonstrating its protective effects against Cr (VI)-induced kidney injury.
This observation suggests that SIMV may have a protective effect against Cr (VI)-induced oxidative stress in rat kidney.
六价铬（Cr(VI)）化合物具有极强的毒性，已证实会在人和动物中引发与氧化应激相关的肾毒性。这些物质在环境中广泛分布，这使得人们对其不良影响的预防作用愈发关注。
本研究旨在确定辛伐他汀（SIMV）对大鼠Cr(VI)诱导的肾毒性的潜在保护作用。
48只成年雄性Wistar大鼠（体重180 - 220克）被随机分为八组（每组n = 6）。第一组给予SIMV 20毫克/千克/天。第二组仅给予赋形剂。第三、五和七组分别腹腔注射（i.p）重铬酸钠（Cr(VI)），剂量为8、12和16毫克/千克体重。第四、六和八组在分别以8、12和16毫克/千克剂量给予Cr(VI)前30分钟，用20毫克/千克的SIMV进行预处理。实验连续进行八天。最后一次给药24小时后，用过量的戊巴比妥钠处死动物。切除肾脏组织以测量丙二醛（MDA）、谷胱甘肽（GSH）并进行组织病理学检查。
铬导致MDA剂量依赖性升高和GSH水平降低。在Cr(VI)处理的大鼠中观察到组织病理学表现。给予SIMV可恢复Cr(VI)在大鼠肾脏中产生的生化和形态学变化。SIMV降低了Cr(VI)处理大鼠的MDA值并提高了GSH水平。SIMV明显逆转了微观损伤，表明其对Cr(VI)诱导的肾损伤具有保护作用。
该观察结果表明，SIMV可能对大鼠肾脏中Cr(VI)诱导的氧化应激具有保护作用。
