Heydari Mojgan, Ahmadizadeh Massumeh, Ahmadi Angali Kambiz
Department of Occupational Health, Engineering, School of Health, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Physiology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
J Nephropathol. 2017 Jul;6(3):168-173. doi: 10.15171/jnp.2017.29. Epub 2016 Dec 20.
1,1,2-Trichloroethylene (TCE) is an important organic solvent which is widespread in the environment. Work place exposure to TCE has been associated adverse effects in many organs including kidney. Vitamin E is an antioxidant that can overcome oxidative stress.
The aim of the present study is to examine the role of vitamin E against destructive effects of TCE on rat kidney.
A total of 35 male Wistar rats were randomly divided into seven groups of equal number in each. The rats in group I were the controls received vehicle only. Animals in groups III, V and VII received intraperitoneal injection (i.p) of corn oil. Rats in groups of II, IV, and VI were received vitamin E at a dose of 200 mg/kg; 30 minutes later, animals were received TCE (i.p) at doses of 1000 mg/kg (groups II and III), 1500 mg/kg (groups of IV and V), and 2000 mg/kg (groups of VI and VII) respectively. The experiment repeated for 7 consecutive days. Twenty-four hours after last administration, animals were killed with overdose of sodium pentobarbital. Blood samples were analyzed for blood urea nitrogen (BUN) and creatinine (Cr). One part of the kidney tissues were excised for measuring malondialdehyde (MDA) and glutathione (GSH) concentrations. Another part were excised for histopathological estimation.
TCE induced a dose-dependent elevation in BUN, Cr, MDA and markedly decreased GSH level when compared to those in control rats. TCE-induced dose-dependent injury in rat kidney tissue. Vitamin E significantly decreased BUN, Cr, MDA and increased GSH levels and protected kidney damage in TCE treated animals.
The observations suggest that vitamin E may have a protective effect against TCE-induced oxidative stress in the rat kidney.
1,1,2-三氯乙烯（TCE）是一种在环境中广泛存在的重要有机溶剂。工作场所接触TCE已被证实会对包括肾脏在内的许多器官产生不良影响。维生素E是一种能够对抗氧化应激的抗氧化剂。
本研究旨在探讨维生素E对TCE所致大鼠肾脏损伤的保护作用。
将35只雄性Wistar大鼠随机分为7组，每组数量相等。第I组大鼠为对照组，仅接受溶剂。第III、V和VII组动物腹腔注射玉米油。第II、IV和VI组大鼠接受200mg/kg剂量的维生素E；30分钟后，分别给予第II和III组1000mg/kg、第IV和V组1500mg/kg、第VI和VII组2000mg/kg剂量的TCE腹腔注射。实验连续进行7天。末次给药24小时后，过量戊巴比妥钠处死动物。检测血样中的血尿素氮（BUN）和肌酐（Cr）。取部分肾组织测定丙二醛（MDA）和谷胱甘肽（GSH）浓度。另取部分进行组织病理学评估。
与对照组大鼠相比，TCE导致BUN、Cr、MDA呈剂量依赖性升高，GSH水平显著降低。TCE对大鼠肾组织造成剂量依赖性损伤。维生素E显著降低了TCE处理动物的BUN、Cr、MDA水平，提高了GSH水平，并保护了肾脏免受损伤。
这些观察结果表明，维生素E可能对TCE诱导的大鼠肾脏氧化应激具有保护作用。
