Blair Cheavar A, Haynes Premi, Campbell Stuart G, Chung Charles, Mitov Mihail I, Dennis Donna, Bonnell Mark R, Hoopes Charles W, Guglin Maya, Campbell Kenneth S
Department of Physiology, University of Kentucky, Lexington, KY.
Department of Pediatrics, Institute for Stem Cell & Regenerative Medicine, University of Washington, Seattle, WA.
VAD J. 2016;2(1). doi: 10.13023/VAD.2016.12. Epub 2016 Jun 3.
This manuscript describes a protocol at the University of Kentucky that allows a translational research team to collect human myocardium that can be used for biological research. We have gained a great deal of practical experience since we started this protocol in 2008, and we hope that other groups might be able to learn from our endeavors. To date, we have procured ~4000 samples from ~230 patients. The tissue that we collect comes from organ donors and from patients who are receiving a heart transplant or a ventricular assist device because they have heart failure. We begin our manuscript by describing the importance of human samples in cardiac research. Subsequently, we describe the process for obtaining consent from patients, the cost of running the protocol, and some of the issues and practical difficulties that we have encountered. We conclude with some suggestions for other researchers who may be considering starting a similar protocol.
本手稿描述了肯塔基大学的一项方案，该方案允许一个转化研究团队收集可用于生物学研究的人类心肌。自2008年启动该方案以来，我们已经获得了大量实践经验，并且希望其他团队能够从我们的努力中有所收获。迄今为止，我们已从约230名患者身上获取了约4000个样本。我们收集的组织来自器官捐献者以及因心力衰竭而接受心脏移植或心室辅助装置的患者。我们在稿件开头阐述了人类样本在心脏研究中的重要性。随后，我们描述了获取患者同意的过程、运行该方案的成本，以及我们遇到的一些问题和实际困难。最后，我们为其他可能考虑启动类似方案的研究人员提供了一些建议。
