Bogdan Agnes, Berta Gergely, Szekeres-Bartho Julia
Department of Medical Biology, Medical School, Pecs, Hungary; János Szentágothai Research Centre, University of Pecs, Hungary; Endocrine Studies, Centre of Excellence, Pecs, Hungary.
Department of Medical Biology, Medical School, Pecs, Hungary.
J Reprod Immunol. 2017 Feb;119:38-43. doi: 10.1016/j.jri.2016.12.001. Epub 2016 Dec 23.
Though uterine NK cells (u NK cells) contain cytotoxic granules, and selectively over- express the genes of perforin and granzymes, during normal pregnancy, they are not cytotoxic. Progesterone is indispensable for the establishment and maintenance of pregnancy both in humans and in mice. Mouse uterine NK cells do not express the classical progesterone receptor, yet progesterone affects the recruitment and function of uterine NK cells, the latter partly via the Progesterone-Induced Blocking Factor (PIBF). We demonstrated PIBF positive granulated cells in the mouse decidua. The aim of this study was to characterize these cells by lectin immunohistochemistry and anti-perforin reactivity. PIBF+ granulated cells were absent from the deciduae of alymphoid mice, but appeared in the decidua of those that had been reconstituted with bone marrow from male BALB/c mice. PIBF+ granulated cells bound the DBA lectin, suggesting their NK cell nature, and also contained perforin, which co-localized with PIBF in the cytoplasmic granules. In anti-progesterone treated mice all of the PIBF+ cells were perforin positive at g. d. 12.5, in contrast to the 54% perforin positivity of PIBF+ cells in untreated mice.
The PIBF+ granulated cells in the decidua belong to the NK population, and PIPB co-localizes with perforin in the cytoplasmic granules.
尽管子宫自然杀伤细胞（uNK细胞）含有细胞毒性颗粒，并选择性地过度表达穿孔素和颗粒酶基因，但在正常妊娠期间，它们并无细胞毒性。孕酮对于人类和小鼠妊娠的建立和维持都是不可或缺的。小鼠子宫自然杀伤细胞不表达经典的孕酮受体，但孕酮会影响子宫自然杀伤细胞的募集和功能，后者部分是通过孕酮诱导阻断因子（PIBF）实现的。我们在小鼠蜕膜中证实了PIBF阳性颗粒细胞。本研究的目的是通过凝集素免疫组织化学和抗穿孔素反应性来表征这些细胞。无淋巴细胞小鼠的蜕膜中不存在PIBF+颗粒细胞，但在用雄性BALB/c小鼠骨髓重建的小鼠蜕膜中出现。PIBF+颗粒细胞结合荆豆凝集素，表明其具有自然杀伤细胞的性质，并且还含有穿孔素，穿孔素与PIBF在细胞质颗粒中共定位。在抗孕酮处理的小鼠中，所有PIBF+细胞在妊娠第12.5天时穿孔素呈阳性，而未处理小鼠中PIBF+细胞的穿孔素阳性率为54%。
蜕膜中的PIBF+颗粒细胞属于自然杀伤细胞群体，且PIPB与穿孔素在细胞质颗粒中共定位。
