Yilmaz Özge Turna, Gündüz Mehmet Can, Evkuran Dal Gamze, Uçmak Melih, Günay Uçmak Zeynep, Karaçam Esra, Kaşikçi Güven, Kiliçarslan Mehmet Ragip
Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Istanbul University, Istanbul, Turkey.
Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Istanbul University, Istanbul, Turkey.
Theriogenology. 2017 Feb;89:183-191. doi: 10.1016/j.theriogenology.2015.09.010. Epub 2015 Sep 11.
We evaluated the changes in the pulsatility index (PI) and resistance index (RI) of fetal and maternal vessels and maternal serum angiogenic factors throughout pregnancy in 20 Kivircik ewes. They were examined for pregnancy detection on Day 30 after mating. The PI and RI during the pregnancies were followed using Doppler ultrasonography (USG) of the uterine artery, umbilical artery, fetal aorta, and fetal vena cava. Doppler USG was performed every 15 days beginning from the 40th day after mating. Blood samples were collected every 15 days starting from the day of mating. Nitric oxide (NO), vascular endothelial growth factor, basic fibroblast growth factor, and angiopoietin-1 and -2 levels were measured in maternal serum. A gradual decline was observed in the PI and RI of the umbilical artery beginning from Day 75 of pregnancy. Similarly, the RI of the uterine artery decreased on Day 135. Embryonic resorption was detected in two ewes with an increased PI and RI in the uterine artery. A significant and gradual decrease in the maternal serum vascular endothelial growth factor level was observed throughout pregnancy. The maternal serum NO level increased beginning from Day 135 of pregnancy. The results of this study illustrate the progressive changes in the Doppler USG findings of fetomaternal vessels and maternal serum angiogenic factors that occur throughout ewe pregnancy. The umbilical and uterine artery Doppler USG findings and maternal serum NO concentration may be important parameters for evaluation of the course of pregnancy in ewes. The results of this study should be compared with those of further studies that include compromised pregnancies and nonpregnant ewes.
我们评估了20只基维里克母羊整个孕期胎儿和母体血管的搏动指数（PI）和阻力指数（RI）以及母体血清血管生成因子的变化。在配种后第30天对它们进行妊娠检测。在整个孕期，使用多普勒超声检查（USG）监测子宫动脉、脐动脉、胎儿主动脉和胎儿腔静脉的PI和RI。从配种后第40天开始，每15天进行一次多普勒超声检查。从配种当天开始，每15天采集一次血样。检测母体血清中的一氧化氮（NO）、血管内皮生长因子、碱性成纤维细胞生长因子以及血管生成素-1和-2的水平。从妊娠第75天开始，观察到脐动脉的PI和RI逐渐下降。同样，子宫动脉的RI在第135天下降。在两只子宫动脉PI和RI升高的母羊中检测到胚胎吸收。在整个孕期观察到母体血清血管内皮生长因子水平显著且逐渐下降。母体血清NO水平从妊娠第135天开始升高。本研究结果表明，在母羊整个孕期，胎儿-母体血管的多普勒超声检查结果和母体血清血管生成因子会发生渐进性变化。脐动脉和子宫动脉的多普勒超声检查结果以及母体血清NO浓度可能是评估母羊妊娠过程的重要参数。本研究结果应与包括妊娠受损母羊和未妊娠母羊的进一步研究结果进行比较。
