Silva Maria Raquel Lopes, Alves Juliana Paula Martins, Fernandes César Carneiro Linhares, Cavalcanti Camila Muniz, Conde Alfredo José Herrera, Bezerra Alessandra Façanha, Soares Anne Caroline Santos, Teixeira Dárcio Ítalo Alves, do Rego Anibal Coutinho, Rondina Davide
School of Veterinary Medicine, Ceará State University (UECE), Fortaleza, Ceará, 60714-903, Brazil.
School of Veterinary Medicine, University of Fortaleza (UNIFOR), Fortaleza, Ceará, 60811-905, Brazil.
Vet World. 2023 Mar;16(3):464-473. doi: 10.14202/vetworld.2023.464-473. Epub 2023 Mar 17.
Despite the wide spectrum of uses, one of the chief drawbacks to expanding microalgae as a food supplement in livestock is the lack of a regimen protocol with established dosage and time length of supplementation. Therefore, this study aimed to investigate the effect of short-term supplementation with increasing doses of microalgae on ovarian response in goats reared in northeast Brazil.
Twenty-eight goats had their follicular waves synchronized using three injections of a prostaglandin analog at 7-day intervals. Goats were allocated to groups that received daily oral Chlorella supplementation for 7 days, respectively: 5 g, GMA5 group (n = 7), 10 g (GMA10; n = 7), and 20 g (GMA20; n = 7). The control group (GMA 0; n = 7) received a drench of water.
The groups showed a quadratic increase (p = 0.0156) in kidney fat thickness but there was a significant reduction in dry matter intake in the GMA20 group. The GMA20 group showed higher glucose levels and glutathione peroxidase (p < 0.05). There was a decrease in plasma cholesterol (p < 0.05) in the 10 and 20 g treatments. The number of total follicles increased quadratically. Follicles <3 mm increased linearly (p = 0.0113) for microalgal supply. The GMA10 and GMA20 groups had the highest values (p < 0.05) among the treatments. After inducing ovulation, there was a significant increase in follicles >3 mm in the GMA10 group, which also showed a greater (p < 0.05) area of intraovarian blood perfusion and pulsatility index of the ovarian artery.
We conclude that for 7 days of supplementation, the administration of 10 g of microalgae appears to be the most efficient dosage for stimulating the ovarian response in tropical goats.
尽管微藻用途广泛，但将其作为家畜食品补充剂进行推广的主要缺点之一是缺乏既定剂量和补充时长的方案协议。因此，本研究旨在调查在巴西东北部饲养的山羊中，短期补充不同剂量微藻对卵巢反应的影响。
28只山羊每隔7天注射三次前列腺素类似物，使卵泡波同步。将山羊分为几组，分别每日口服小球藻补充剂7天：5克，GMA5组（n = 7）、10克（GMA10；n = 7）和20克（GMA20；n = 7）。对照组（GMA 0；n = 7）给予灌服水。
各组肾脂肪厚度呈二次增加（p = 0.0156），但GMA20组干物质摄入量显著降低。GMA20组葡萄糖水平和谷胱甘肽过氧化物酶较高（p < 0.05）。10克和20克处理组血浆胆固醇降低（p < 0.05）。卵泡总数呈二次增加。微藻供应使直径<3毫米的卵泡呈线性增加（p = 0.0113）。GMA10组和GMA20组在各处理组中值最高（p < 0.05）。诱导排卵后，GMA10组直径>3毫米的卵泡显著增加，其卵巢内血液灌注面积和卵巢动脉搏动指数也更大（p < 0.05）。
我们得出结论，对于7天的补充期，给予10克微藻似乎是刺激热带山羊卵巢反应的最有效剂量。
