Atta-ur-Rahman Institute for Natural Product Discovery, Universiti Teknologi MARA (UiTM), Puncak Alam Campus, 42300 Bandar Puncak Alam, Selangor D.E., Malaysia; Faculty of Applied Science, UiTM Shah Alam, 40450 Shah Alam, Selangor D.E., Malaysia.
Atta-ur-Rahman Institute for Natural Product Discovery, Universiti Teknologi MARA (UiTM), Puncak Alam Campus, 42300 Bandar Puncak Alam, Selangor D.E., Malaysia; Faculty of Applied Science, UiTM Shah Alam, 40450 Shah Alam, Selangor D.E., Malaysia.
Bioorg Chem. 2017 Feb;70:184-191. doi: 10.1016/j.bioorg.2016.12.009. Epub 2016 Dec 26.
Thiourea derivatives having benzimidazole 1-17 have been synthesized, characterized by H NMR, C NMR and EI-MS and evaluated for α-glucosidase inhibition. Identification of potential α-glucosidase inhibitors were done by in vitro screening of 17 thiourea bearing benzimidazole derivatives using Baker's yeast α-glucosidase enzyme. Compounds 1-17 exhibited a varying degree of α-glucosidase inhibitory activity with IC values between 35.83±0.66 and 297.99±1.20μM which are more better than the standard acarbose (IC=774.5±1.94μM). Compound 10 and 14 showed significant inhibitory effects with IC value 50.57±0.81 and 35.83±0.66μM, respectively better than the rest of the series. Structure activity relationships were established. Molecular docking studies were performed to understand the binding interaction of the compounds.
合成了具有苯并咪唑 1-17 的硫脲衍生物，通过 H NMR、C NMR 和 EI-MS 进行了表征，并评估了它们对α-葡萄糖苷酶的抑制作用。通过使用贝克氏酵母α-葡萄糖苷酶对 17 个含有苯并咪唑的硫脲衍生物进行体外筛选，确定了潜在的α-葡萄糖苷酶抑制剂。化合物 1-17 表现出不同程度的α-葡萄糖苷酶抑制活性，IC 值在 35.83±0.66 和 297.99±1.20μM 之间，优于标准阿卡波糖（IC=774.5±1.94μM）。化合物 10 和 14 表现出显著的抑制作用，IC 值分别为 50.57±0.81 和 35.83±0.66μM，优于其他化合物。建立了构效关系。进行了分子对接研究，以了解化合物的结合相互作用。
