Zabel M, Schäfer H
Department of Histology and Embryology, Academy of Medicine, Poznań, Poland.
Bone Miner. 1989 Sep;7(2):107-12. doi: 10.1016/0169-6009(89)90068-8.
The presence of the 28K vitamin D-dependent, calcium-binding protein (28K calbindin) was investigated by immunocytochemistry in normal thyroid glands and parathyroid glands of rats, guinea pigs, rabbits and men, as well as in human thyroid medullary carcinomas and human parathyroid adenomas. In addition, thyroid glands and parathyroid glands of rats and guinea pigs were studied after treatment with vitamin D3 injected intramuscularly at a total dose of 1.2 x 10(6) IU per 100 g body weight. 28K calbindin was found exclusively in parafollicular cells of guinea pigs and never in those of other species investigated. It was present predominantly in the cytoplasm and in lower concentration in nuclei. After vitamin D3 treatment, increased immunoreactivity of 28K calbindin was observed in the cytoplasm and, even more pronounced, in the nuclei. In normal parathyroid cells and in parathyroid tumors and medullary thyroid carcinomas, 28K calbindin was not demonstrable. Our findings suggest an important function of calbindin in the cellular calcium processing of parafollicular cells of guinea pigs.
采用免疫细胞化学方法，研究了28K维生素D依赖性钙结合蛋白（28K钙结合蛋白）在大鼠、豚鼠、兔和人的正常甲状腺及甲状旁腺中的表达情况，以及在人甲状腺髓样癌和甲状旁腺腺瘤中的表达情况。此外，对大鼠和豚鼠肌肉注射维生素D3，总剂量为每100g体重1.2×10(6)IU，之后研究其甲状腺和甲状旁腺。结果发现，28K钙结合蛋白仅存在于豚鼠的滤泡旁细胞中，在所研究的其他物种的滤泡旁细胞中均未发现。它主要存在于细胞质中，细胞核中的浓度较低。维生素D3处理后，在细胞质中观察到28K钙结合蛋白的免疫反应性增强，在细胞核中更为明显。在正常甲状旁腺细胞、甲状旁腺肿瘤和甲状腺髓样癌中，未检测到28K钙结合蛋白。我们的研究结果表明，钙结合蛋白在豚鼠滤泡旁细胞的细胞钙处理过程中具有重要作用。
