Zabel M, Schäfer H
Department of Histology and Embryology, Academy of Medicine, Poznań, Poland.
Histochemistry. 1988;88(3-6):623-8. doi: 10.1007/BF00570333.
Hypercalcemia was induced in rats by the administration of A.T.10. We then determined the levels of total and ionized calcium and calcitonin in the serum, as well as performed ultrastructural observations and histochemical investigations of the calcitonin and neuron-specific enolase immunoreactivities in the stimulated parafollicular cells. The main aim of the study was to apply histochemical procedures to determine the immunoreactions of calcitonin gene-related peptide (CGRP), somatostatin and secretory protein-I in stimulated parafollicular cells. Immunoreactions of CGRP and calcitonin decreased strikingly in A.T.10-treated animals, whereas no visible changes were noted in somatostatin immunoreactivity. In the case of secretory protein-I, an insignificant increase of its immunoreactivity was observed in the treated animals. The cytophysiological significance of these results is discussed.
通过给予A.T.10诱导大鼠出现高钙血症。然后我们测定了血清中总钙、离子钙和降钙素的水平，并对刺激后的滤泡旁细胞中降钙素和神经元特异性烯醇化酶免疫反应性进行了超微结构观察和组织化学研究。该研究的主要目的是应用组织化学方法来确定刺激后的滤泡旁细胞中降钙素基因相关肽（CGRP）、生长抑素和分泌蛋白-I的免疫反应。在接受A.T.10治疗的动物中，CGRP和降钙素的免疫反应显著降低，而生长抑素免疫反应性未见明显变化。在分泌蛋白-I方面，在接受治疗的动物中观察到其免疫反应性有不显著的增加。讨论了这些结果的细胞生理学意义。
