Department of Geriatrics, Beijing Hospital, National Center of Gerontology, Beijing, The People's Republic of China.
Department of Laboratory Medicine, Beijing Hospital, Beijing, The People's Republic of China.
Sci Rep. 2017 Jan 3;7:39606. doi: 10.1038/srep39606.
Invasive fungal infections acquired in the hospital have progressively emerged as an important cause of life-threatening infection. In particular, airborne fungi in hospitals are considered critical pathogens of hospital-associated infections. To identify the causative airborne microorganisms, high-volume air samplers were utilized for collection, and species identification was performed using a culture-based method and DNA sequencing analysis with the Illumina MiSeq and HiSeq 2000 sequencing systems. Few bacteria were grown after cultivation in blood agar. However, using microbiome sequencing, the relative abundance of fungi, Archaea species, bacteria and viruses was determined. The distribution characteristics of fungi were investigated using heat map analysis of four departments, including the Respiratory Intensive Care Unit, Intensive Care Unit, Emergency Room and Outpatient Department. The prevalence of Aspergillus among fungi was the highest at the species level, approximately 17% to 61%, and the prevalence of Aspergillus fumigatus among Aspergillus species was from 34% to 50% in the four departments. Draft genomes of microorganisms isolated from the hospital environment were obtained by sequence analysis, indicating that investigation into the diversity of airborne fungi may provide reliable results for hospital infection control and surveillance.
在医院获得的侵袭性真菌感染已逐渐成为危及生命的感染的重要原因。特别是，医院空气中的真菌被认为是医院相关感染的关键病原体。为了鉴定致病的空气传播微生物，使用大容量空气采样器进行采集，并使用基于培养的方法和 Illumina MiSeq 和 HiSeq 2000 测序系统的 DNA 测序分析进行物种鉴定。在血液琼脂中培养后很少有细菌生长。然而，使用微生物组测序，可以确定真菌、古菌、细菌和病毒的相对丰度。通过对包括呼吸重症监护病房、重症监护病房、急诊室和门诊部在内的四个部门的热图分析，研究了真菌的分布特征。在真菌中，曲霉菌的患病率最高，约为 17%至 61%，在四个部门中，烟曲霉菌在曲霉菌种中的患病率为 34%至 50%。通过序列分析获得了从医院环境中分离出的微生物的基因组草图，这表明对空气传播真菌的多样性进行调查可能为医院感染控制和监测提供可靠的结果。
