Center for Biomedical Imaging, Department of Radiology, New York University School of Medicine, New York, NY, 10012, USA.
Chamberlain Laboratory, University of Washington, Department of Neurology, Seattle, Washington, 98195, USA.
Sci Rep. 2017 Jan 3;7:39496. doi: 10.1038/srep39496.
Recently, the first magnetic resonance microscopy (MRM) images at the cellular level in isolated mammalian brain tissues were obtained using microsurface coils. These methods can elucidate the cellular origins of MR signals and describe how these signals change over the course of disease progression and therapy. In this work, we explore the capability of these microimaging techniques to visualize mouse muscle fibers and their nuclei. Isolated myofibers expressing lacZ were imaged with and without a stain for β-galactosidase activity (S-Gal + ferric ammonium citrate) that produces both optical and MR contrast. We found that MRM can be used to image single myofibers with 6-μm resolution. The ability to image single myofibers will serve as a valuable tool to study MR properties attributed to healthy and myopathic cells. The ability to image nuclei tagged with MR/Optical gene markers may also find wide use in cell lineage MRI studies.
最近，使用微表面线圈在分离的哺乳动物脑组织中获得了细胞水平的第一个磁共振显微镜 (MRM) 图像。这些方法可以阐明 MR 信号的细胞起源，并描述这些信号在疾病进展和治疗过程中的变化。在这项工作中，我们探索了这些微成像技术可视化小鼠肌肉纤维及其核的能力。表达 lacZ 的分离肌纤维用和不用β-半乳糖苷酶活性染色剂（S-Gal+柠檬酸铁铵）进行成像，该染色剂产生光学和磁共振对比。我们发现，MRM 可用于以 6μm 的分辨率成像单根肌纤维。成像单根肌纤维的能力将成为研究归因于健康和肌病细胞的磁共振特性的有价值工具。用磁共振/光学基因标记物标记核的成像能力也可能在细胞谱系 MRI 研究中得到广泛应用。
