Nielsen K, Orntoft T, Wolf H
Institute of Pathology, Aalborg Hospital, Denmark.
Cancer. 1989 Dec 1;64(11):2269-74. doi: 10.1002/1097-0142(19891201)64:11<2269::aid-cncr2820641115>3.0.co;2-u.
The mean nuclear volume of cells in bladder tumors was estimated using the principle for estimation of the volume of particles of arbitrary shape. The study included three groups of patients: one group of 10 patients with only one nonrecurrent, noninvasive bladder tumor (Ta), another group of 11 patients with recurrent noninvasive bladder tumors, and a third group of 14 patients with recurrent primary noninvasive bladder tumors who ultimately developed an invasive tumor. After standard fixation, embedding, sectioning, and hematoxylin-eosin staining an unbiased estimate of the mean volume of nuclei sampled with a chance proportional to their volume was calculated: nu v = pi/3.l3(0). Here lo is the length of the intercept through a test point hitting a nucleus measured in a random direction through the test point. In the primary tumor the mean, nuclear volume of the cells in tumors from patients with a single bladder tumor is small (141 micrograms 3 geometric mean) with only one of ten above 165 microns 3. The mean nuclear volume of the primary tumor cells in patients with recurrent noninvasive bladder tumors was slightly larger (195 microns 3 geometric mean) with five of 11 above 165 microns 3. The mean nuclear volume of the primary tumor cells in patients who ended up with invasive tumors was higher (245 microns 3 geometric mean) with 12 of 14 above 165 microns 3. A large mean nuclear volume of cells in noninvasive bladder tumors (Ta) indicates recurrence and an invasive potential. Considering that nearly all primary tumors were classified as Bergkvist Grade II tumors, the prognostic precision of absolute mean nuclear volume is noticeable. This simple and fast estimate of mean nuclear volume seems to provide objective data with high prognostic value in primary noninvasive bladder tumors (Ta).
采用估算任意形状颗粒体积的原理，对膀胱肿瘤细胞的平均核体积进行估算。该研究纳入了三组患者：一组为10例仅患有一个非复发性、非浸润性膀胱肿瘤（Ta期）的患者；另一组为11例患有复发性非浸润性膀胱肿瘤的患者；第三组为14例患有复发性原发性非浸润性膀胱肿瘤且最终发展为浸润性肿瘤的患者。在进行标准固定、包埋、切片和苏木精-伊红染色后，计算与细胞核体积成比例抽取样本的平均体积的无偏估计值：核体积（nu v）=π/3·l3(0)。这里的l0是通过测试点并在随机方向穿过测试点时与细胞核相交的截距长度。在原发性肿瘤中，患有单个膀胱肿瘤患者的肿瘤细胞平均核体积较小（几何平均值为141立方微米），10例中只有1例高于165立方微米。患有复发性非浸润性膀胱肿瘤患者的原发性肿瘤细胞平均核体积略大（几何平均值为195立方微米），11例中有5例高于165立方微米。最终发展为浸润性肿瘤患者的原发性肿瘤细胞平均核体积更高（几何平均值为245立方微米），14例中有12例高于165立方微米。非浸润性膀胱肿瘤（Ta期）细胞的平均核体积较大表明有复发和浸润潜能。鉴于几乎所有原发性肿瘤都被归类为伯格维斯特二级肿瘤，绝对平均核体积的预后准确性较为显著。这种对平均核体积的简单快速估算似乎能为原发性非浸润性膀胱肿瘤（Ta期）提供具有高预后价值的客观数据。
