Nielsen K, Colstrup H, Nilsson T, Gundersen H J
Virchows Arch B Cell Pathol Incl Mol Pathol. 1986;52(1):41-54. doi: 10.1007/BF02889949.
The aim of this retrospective study is to provide morphometric data which make grading of urinary bladder tumours objective and reproducible by stereological estimation of nuclear volume using the principle of estimating of the volume of particles of arbitrary shape. The study includes 92 specimens: 12 from normal bladder mucosa, and 80 from bladder tumours (15 grade I, 45 grade II, 19 grade III and one grade IV according to Bergkvist et al. 1965). After standard fixation, embedding, sectioning and hematoxylin-eosin staining, an unbiased estimate of the mean volume of nuclei sampled with a change proportional to the volume: (Formula: see text) was calculated. Here l0 is the length of the intercept through a test point hitting a nucleus measured in a random direction through the test point. The weighted means of nuclear volume in bladder tumours are spread over a wide range and show a strong correlation with the Bergkvist grade. Moreover, the relationship between the weighted mean volume of nuclei in bladder tumours and the prognosis is very good. Only one of 35 patients with a mean nuclear volume below 300 micron 3 died of bladder cancer whereas 18 of 19 patients with a mean nuclear volume above 500 micron 3 developed invasives tumours or died of their disease. This simple and fast estimate of nuclear volume seems to provide objective data of high prognostic value.
这项回顾性研究的目的是提供形态计量学数据，通过使用估计任意形状颗粒体积的原理对细胞核体积进行体视学估计，使膀胱肿瘤的分级客观且可重复。该研究包括92个标本：12个来自正常膀胱黏膜，80个来自膀胱肿瘤（根据Bergkvist等人1965年的标准，15个为I级，45个为II级，19个为III级，1个为IV级）。经过标准固定、包埋、切片和苏木精-伊红染色后，计算了与体积成比例变化采样的细胞核平均体积的无偏估计值：（公式：见正文）。这里l0是通过测试点在随机方向上穿过细胞核时测得的截距长度。膀胱肿瘤细胞核体积的加权平均值分布范围很广，与Bergkvist分级有很强的相关性。此外，膀胱肿瘤细胞核加权平均体积与预后之间的关系非常好。细胞核平均体积低于300立方微米的35例患者中只有1例死于膀胱癌，而细胞核平均体积高于500立方微米的19例患者中有18例发展为浸润性肿瘤或死于该疾病。这种简单快速的细胞核体积估计似乎提供了具有高预后价值的客观数据。
