Nielsen K, Ostri P
Institute of Pathology, Gentofte Hospital, University of Copenhagen, Hellerup, Denmark.
APMIS Suppl. 1988;4:87-91.
The aim of this retrospective study was to provide quantitative data which make grading of primary tumors of the renal pelvis objective and reproducible by stereological estimation of nuclear volume using the principle of estimation of the mean volume of particles of arbitrary shape. The study includes 35 kidney specimens with primary transitional cell tumor of the renal pelvis. After standard fixation, embedding, sectioning and hematoxylin-eosin staining, an unbiased estimate of the mean volume of nuclei sampled with a change proportional to volume: (formula; see text) was calculated. Here 1(0) is the length of the intercept through a test point hitting a nucleus measured in a random direction. The mean nuclear volumes of the tumors are spread over a wide range, from 159 microns 3 to 1.555 microns 3. Ten of 11 patients with nuclear volume above 800 microns 3 died of the disease while only 8 of 24 patients with a mean nuclear volume below 800 microns 3 died of the disease. This simple and fast estimate of mean nuclear volume seems to provide objective data with a high prognostic value.
这项回顾性研究的目的是提供定量数据，通过使用任意形状颗粒平均体积估计原理对细胞核体积进行体视学估计，使肾盂原发性肿瘤的分级客观且可重复。该研究包括35例患有肾盂原发性移行细胞肿瘤的肾脏标本。经过标准固定、包埋、切片和苏木精-伊红染色后，计算了与体积成比例变化采样的细胞核平均体积的无偏估计值：（公式；见正文）。这里1(0)是在随机方向上穿过一个击中细胞核的测试点的截距长度。肿瘤的平均细胞核体积分布范围很广，从159立方微米到1555立方微米。11例平均细胞核体积高于800立方微米的患者中有10例死于该疾病，而24例平均细胞核体积低于800立方微米的患者中只有8例死于该疾病。这种对平均细胞核体积的简单快速估计似乎能提供具有高预后价值的客观数据。
