Nielsen K
Institute of Pathology, University of Copenhagen, Gentofte Hospital, Hellerup, Denmark.
Virchows Arch B Cell Pathol Incl Mol Pathol. 1988;55(4):233-6. doi: 10.1007/BF02896580.
The nuclear volume of the epithelial cells in the human urinary bladder mucosa has been estimated using point sampled intercepts in vertical sections (local vertical windows). The study included 27 specimens: ten from normal bladder mucosa, five from inflamed mucosa, seven from mucosa with flat grade II lesions and five from mucosa with flat grade III lesions. After standard fixation, embedding, sectioning and haematoxylin-eosin staining an unbiased estimate of the mean volume of nuclei sampled with a chance proportion to the volume = vV = pi/3 x (l0)3 was calculated using a frame for orientating the linear test probe in vertical sections. Here l0 is the length of the intercept through a test point hitting a nucleus measured in a random direction through the test point. The weighted mean nuclear volume of bladder mucosa with grade II and grade III lesions (537 microns 3 and 494 microns 3 respectively) was significantly larger than the weighted mean nuclear volume of normal (133 microns 3) and inflamed bladder mucosa (182 microns 3). This simple and fast estimation of nuclear volume seems to provide objective data useful in discriminating between neoplastic and non-neoplastic lesions of the bladder mucosa.
利用垂直切片（局部垂直窗口）中的点抽样截距法，对人膀胱黏膜上皮细胞的核体积进行了估计。该研究纳入了27个标本：10个来自正常膀胱黏膜，5个来自炎症黏膜，7个来自伴有扁平二级病变的黏膜，5个来自伴有扁平三级病变的黏膜。在进行标准固定、包埋、切片及苏木精-伊红染色后，使用一个用于在垂直切片中定位线性测试探针的框架，计算出与体积成机会比例抽样的细胞核平均体积的无偏估计值(vV = \pi/3×(l0)^3)。这里(l0)是通过测试点在随机方向上穿过细胞核的截距长度。伴有二级和三级病变的膀胱黏膜的加权平均核体积（分别为537立方微米和494立方微米）显著大于正常膀胱黏膜（133立方微米）和炎症膀胱黏膜（182立方微米）的加权平均核体积。这种简单快速的核体积估计似乎能提供有助于区分膀胱黏膜肿瘤性和非肿瘤性病变的客观数据。
