Department of Pharmacy, COMSATS Institute of Information Technology, 22060, Abbottabad, Pakistan.
Department of Biochemistry and Biotechnology, Islamia University of Bahawalpur, 63100, Bahawalpur, Pakistan.
BMC Complement Altern Med. 2017 Jan 3;17(1):3. doi: 10.1186/s12906-016-1511-4.
Gaultheria trichophylla (Royle) is used as food and for treating many ailments in folk medicine especially against inflammation. The purpose of this in vitro study was to evaluate the ability of extracts of G. trichophylla as anti-oxidant and anti-inflammatory agent and for its mineral contents.
Powdered plant material (100 g) was extracted with 100 ml each of methanol, chloroform, and n-hexane using soxhlet extractor. Antioxidant activity of methanol extract was assessed by DPPH radical scavenging and FRAP assays. Determination of enzyme inhibition activity was determined using 5-LOX inhibitory activity. Total phenolic and flavonoids contents were measured by Folin-Chicalteu and colorimeteric methods respectively. Minerals and heavy metals contents were determined using Atomic absorption spectrophotometer. Qualitative HPLC analysis were performed using some standard phenolic compounds.
The highest phenolic (17.5 ± 2.5 mg GA equivalent/g) and flavonoids (41.3 ± 0.1 mg QE equivalent/g) concentrations were found in methanol extract, which also showed more scavenging activity of 1, 1-diphenyl-2-picrylhydrazyl and ferrous reducing power with IC = 81.2 ± 0.2 and IC = 11.2 ± 0.1 μg/ml, respectively. The methanol and chloroform extracts showed best inhibition of 5-lipoxygenase enzyme with 90.5 ± 0.7% and 66.9 ± 0.1% at 0.5 mg/ml, respectively. G. trichophylla extract was also evaluated for mineral contents (K, Na, Ca, Mg, Fe, and Cu), and for chemical profiling of heavy metals (Cr, Pb, Cd, Co, Zn, Ni and Hg).
Our current findings suggest that this plant is good source of minerals and concentration of all heavy metals were within permissible limits. The results revealed that this ignored plant has great pharmaceutical and nutraceutical potential.
Gaultheria trichophylla（罗叶）在民间医学中被用作食物和治疗多种疾病，特别是针对炎症。本体外研究的目的是评估 G. trichophylla 提取物作为抗氧化和抗炎剂及其矿物质含量的能力。
使用索氏提取器用 100ml 甲醇、氯仿和正己烷分别提取植物粉末（100g）。通过 DPPH 自由基清除和 FRAP 测定评估甲醇提取物的抗氧化活性。通过 5-脂氧合酶抑制活性测定确定酶抑制活性。通过福林-希卡尔特和比色法分别测量总酚和类黄酮含量。使用原子吸收分光光度计测定矿物质和重金属含量。使用一些标准酚类化合物进行高效液相色谱分析的定性分析。
甲醇提取物中发现最高的酚类（17.5±2.5mgGA 当量/g）和类黄酮（41.3±0.1mgQE 当量/g）浓度，其对 1,1-二苯基-2-苦基肼和亚铁还原能力的清除活性也分别为 IC=81.2±0.2 和 IC=11.2±0.1μg/ml。甲醇和氯仿提取物对 5-脂氧合酶的抑制作用最好，在 0.5mg/ml 时分别为 90.5±0.7%和 66.9±0.1%。还评估了 Gaultheria trichophylla 提取物的矿物质含量（K、Na、Ca、Mg、Fe 和 Cu）和重金属（Cr、Pb、Cd、Co、Zn、Ni 和 Hg）的化学特征。
我们目前的研究结果表明，这种植物是矿物质的良好来源，所有重金属的浓度都在允许范围内。研究结果表明，这种被忽视的植物具有很大的药用和营养潜力。
