Aquaporin-3 mediates hydrogen peroxide-dependent responses to environmental stress in colonic epithelia.

The colonic epithelium provides an essential barrier against the environment that is critical for protecting the body and controlling inflammation. In response to injury or gut microbes, colonic epithelial cells produce extracellular hydrogen peroxide (HO), which acts as a potent signaling molecule affecting barrier function and host defense. In humans, impaired regulation of HO in the intestine has been associated with early-onset inflammatory bowel disease and colon cancer. Here, we show that signal transduction by HO depends on entry into the cell by transit through aquaporin-3 (AQP3), a plasma membrane HO-conducting channel. In response to injury, AQP3-depleted colonic epithelial cells showed defective lamellipodia, focal adhesions, and repair after wounding, along with impaired HO responses after exposure to the intestinal pathogen Citrobacter rodentium Correspondingly, AQP3 mice showed impaired healing of superficial wounds in the colon and impaired mucosal innate immune responses against C. rodentium infection, manifested by reduced crypt hyperplasia, reduced epithelial expression of IL-6 and TNF-α, and impaired bacterial clearance. These results elucidate the signaling mechanism of extracellular HO in the colonic epithelium and implicate AQP3 in innate immunity at mucosal surfaces.