Srinivas Banoth, Rani Ganapathiwar Swarupa, Kumar Bhukya Kiran, Chandrasekhar Banoth, Krishna Kommalapati Vamsi, Devi Tangutur Anjana, Bhima Bhukya
Department of Microbiology, University College of Science, Osmania University, Hyderabad, Telangana, 500007, India.
Center for Chemical Biology, CSIR-Indian Institute of Chemical Technology, Tarnaka, Hyderabad, Telangana, 500007, India.
AMB Express. 2017 Dec;7(1):2. doi: 10.1186/s13568-016-0301-1. Epub 2017 Jan 3.
The purpose of this study is to evaluate the probiotic characteristics of 15 yeast strains isolated from nectar of toddy palm. Initially, the collected samples were inoculated on yeast extract peptone dextrose agar plates and the colonies so obtained were culturally and morphologically characterized. Commercial probiotic yeast, Saccharomyces boulardii served as the control in these experiments. Of the 15 yeast strains, the isolates that were resistant to antibiotics and worked synergistically with other cultures were considered for further evaluation. Selected isolates were evaluated in vitro for tolerance to simulated gastrointestinal conditions such as temperature, pH, bile and gastric juice. Further the yeast isolates were evaluated for their pathogenicity and adherence to intestinal epithelial cells. The 2 yeast isolates with efficient probiotic properties were finally characterized by sequencing their 5.8 S rRNA and partial sequences of internal transcribed spacer 1 and 2. The sequences were BLAST searched in the National Center for Biotechnology Information, nucleic acid database for sequence similarity of organisms and phylogenetic evolutionary analysis was carried out. Based on maximum similarity of basic local alignment search tool results, organisms were characterized as Pichia kudriavzevii OBS1 (100%) and Saccharomyces cerevisiae OBS2 (96%) and sequences were finally deposited in the GenBank data library. Among these two isolates, S. cerevisiae OBS2 displayed slight/moderate antioxidant and anticancer property. Hence, strain OBS2 can be utilized and explored as a potential probiotic for therapeutic applications.
本研究的目的是评估从棕榈酒花蜜中分离出的15株酵母菌株的益生菌特性。最初，将收集的样品接种在酵母提取物蛋白胨葡萄糖琼脂平板上，对获得的菌落进行培养和形态学特征分析。商业益生菌酵母布拉酵母菌在这些实验中作为对照。在这15株酵母菌株中，对抗生素具有抗性并能与其他培养物协同作用的分离株被考虑进一步评估。对选定的分离株进行体外评估，以检测其对模拟胃肠道条件（如温度、pH值、胆汁和胃液）的耐受性。此外，还对酵母分离株的致病性和对肠上皮细胞的黏附性进行了评估。最后，通过对具有高效益生菌特性的2株酵母分离株的5.8 S rRNA以及内部转录间隔区1和2的部分序列进行测序来进行特征分析。将这些序列在国家生物技术信息中心的核酸数据库中进行BLAST搜索，以寻找生物序列相似性，并进行系统发育进化分析。根据基本局部比对搜索工具结果的最大相似性，将这些生物鉴定为库德里阿兹威毕赤酵母OBS1（100%）和酿酒酵母OBS2（96%），最终将序列存入GenBank数据库。在这两株分离株中，酿酒酵母OBS2表现出轻微/中度的抗氧化和抗癌特性。因此，菌株OBS2可作为一种潜在的益生菌用于治疗应用进行利用和探索。
