Zahoor Farah, Sooklim Chayaphathra, Songdech Pattanan, Duangpakdee Orawan, Soontorngun Nitnipa
Biochemical Technology Division, School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, Bangkok 10150, Thailand.
Native Honeybee and Pollinator Research Center, Ratchaburi Campus, King Mongkut's University of Technology Thonburi, Rang Bua, Chom Bueng, Ratchaburi 70150, Thailand.
Metabolites. 2021 May 13;11(5):312. doi: 10.3390/metabo11050312.
Excessive use of antibiotics has detrimental consequences, including antibiotic resistance and gut microbiome destruction. Probiotic-rich diets help to restore good microbes, keeping the body healthy and preventing the onset of chronic diseases. Honey contains not only prebiotic oligosaccharides but, like yogurt and fermented foods, is an innovative natural source for probiotic discovery. Here, a collection of three honeybee samples was screened for yeast strains, aiming to characterize their potential in vitro probiotic properties and the ability to produce valuable metabolites. Ninety-four isolates out of one-hundred and four were able to grow at temperatures of 30 °C and 37 °C, while twelve isolates could grow at 42 °C. Fifty-eight and four isolates displayed the ability to grow under stimulated gastrointestinal condition, at pH 2.0-2.5, 0.3% (/) bile salt, and 37 °C. Twenty-four isolates showed high autoaggregation of 80-100% and could utilize various sugars, including galactose and xylose. The cell count of these isolates (7-9 log cfu/mL) was recorded and stable during 6 months of storage. Genomic characterization based on the internal transcribed spacer region (ITS) also identified four isolates of displayed good ability to produce antimicrobial acids. These results provided the basis for selecting four natural yeast isolates as starter cultures for potential probiotic application in functional foods and animal feed. Additionally, these isolates also produced high levels of acids from fermented sugarcane molasses, an abundant agricultural waste product from the sugar industry. Furthermore, one of ten identified isolates of displayed an excellent ability to produce a pentose sugar xylitol at a yield of 0.490 g/g of consumed xylose. Potentially, yeast isolates of honeybee samples may offer various biotechnological advantages as probiotics or metabolite producers of multiproduct-based lignocellulosic biorefinery.
过度使用抗生素会产生有害后果，包括抗生素耐药性和肠道微生物群破坏。富含益生菌的饮食有助于恢复有益微生物，保持身体健康并预防慢性病的发生。蜂蜜不仅含有益生元低聚糖，而且与酸奶和发酵食品一样，是发现益生菌的创新天然来源。在此，对三个蜜蜂样本的集合进行酵母菌株筛选，旨在表征它们在体外的潜在益生菌特性以及产生有价值代谢物的能力。104个分离株中有94个能够在30°C和37°C的温度下生长，而12个分离株能够在42°C下生长。58个和4个分离株显示出在模拟胃肠道条件下（pH 2.0 - 2.5、0.3%（/）胆盐和37°C）生长的能力。24个分离株显示出80 - 100%的高自聚集能力，并且能够利用包括半乳糖和木糖在内的各种糖类。记录了这些分离株的细胞计数（7 - 9 log cfu/mL），并且在储存6个月期间保持稳定。基于内部转录间隔区（ITS）的基因组表征还鉴定出四个分离株具有良好的产生抗菌酸的能力。这些结果为选择四种天然酵母分离株作为潜在益生菌应用于功能性食品和动物饲料的起始培养物提供了依据。此外，这些分离株还能从甘蔗废糖蜜（制糖工业丰富的农业废弃物）发酵中产生高水平的酸。此外，在鉴定出的十个分离株中，有一个显示出以0.490 g/g消耗的木糖的产量产生戊糖木糖醇的优异能力。潜在地，蜜蜂样本的酵母分离株作为益生菌或基于多产品的木质纤维素生物精炼厂的代谢物生产者可能具有各种生物技术优势。
