黄褐纤维单胞菌的木聚糖酶：表达、生化特性及生物技术潜力

Xylanases of Cellulomonas flavigena: expression, biochemical characterization, and biotechnological potential.

作者信息

Lisov Alexander V, Belova Oksana V, Lisova Zoya A, Vinokurova Nataliy G, Nagel Alexey S, Andreeva-Kovalevskaya Zhanna I, Budarina Zhanna I, Nagornykh Maxim O, Zakharova Marina V, Shadrin Andrey M, Solonin Alexander S, Leontievsky Alexey A

机构信息

G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences (IBPM RAS), 5 Prospekt Nauki, Pushchino, Moscow Region, 142290, Russia.

Pushchino State Institute of Life Sciences, 3 Prospekt Nauki, Pushchino, Moscow Region, 142290, Russia.

出版信息

AMB Express. 2017 Dec;7(1):5. doi: 10.1186/s13568-016-0308-7. Epub 2017 Jan 3.

Four xylanases of Cellulomonas flavigena were cloned, expressed in Escherichia coli and purified. Three enzymes (CFXyl1, CFXyl2, and CFXyl4) were from the GH10 family, while CFXyl3 was from the GH11 family. The enzymes possessed moderate temperature stability and a neutral pH optimum. The enzymes were more stable at alkaline pH values. CFXyl1 and CFXyl2 hydrolyzed xylan to form xylobiose, xylotriose, xylohexaose, xylopentaose, and xylose, which is typical for GH10. CFXyl3 (GH11) and CFXyl4 (GH10) formed the same xylooligosaccharides, but xylose was formed in small amounts. The xylanases made efficient saccharification of rye, wheat and oat, common components of animal feed, which indicates their high biotechnological potential.