Eida Abdul Aziz, Bougouffa Salim, Alam Intikhab, Hirt Heribert, Saad Maged M
DARWIN21, Center for Desert Agriculture (CDA), King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900, Saudi Arabia.
Computational Bioscience Research Center (CBRC), King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900, Saudi Arabia.
Curr Microbiol. 2021 Apr;78(4):1135-1141. doi: 10.1007/s00284-021-02429-5. Epub 2021 Mar 8.
Cellulomonas sp. JZ18 is a gram-positive, rod shaped bacterium that was previously isolated from the root endosphere of the perennial desert tussock-grass Panicum turgidum. Genome coverage of PacBio sequencing was approximately 199X. Genome assembly generated a single chromosome of 7,421,843 base pairs with a guanine-cytosine (GC) content of 75.60% with 3240 protein coding sequences, 361 pseudo genes, three ribosomal RNA operons, three non-coding RNAs and 45 transfer RNAs. Comparison of JZ18's genome with type strains from the same genus, using digital DNA-DNA hybridization and average nucleotide identity calculations, revealed that JZ18 might potentially belong to a new species. Functional analysis revealed the presence of genes that may complement previously observed biochemical and plant phenotypes. Furthermore, the presence of a number of enzymes could be of potential use in industrial processes as biocatalysts. Genome sequencing and analysis, coupled with comparative genomics, of endophytic bacteria for their potential plant growth promoting activities under different soil conditions will accelerate the knowledge and applications of biostimulants in sustainable agriculture.
纤维单胞菌JZ18是一种革兰氏阳性杆菌，先前从多年生沙漠丛生草胀果黍的根内皮层中分离得到。PacBio测序的基因组覆盖度约为199倍。基因组组装产生了一条7,421,843个碱基对的单染色体，鸟嘌呤-胞嘧啶（GC）含量为75.60%，有3240个蛋白质编码序列、361个假基因、三个核糖体RNA操纵子、三个非编码RNA和45个转运RNA。通过数字DNA-DNA杂交和平均核苷酸同一性计算，将JZ18的基因组与同一属的模式菌株进行比较，结果表明JZ18可能属于一个新物种。功能分析表明存在一些基因，这些基因可能补充先前观察到的生化和植物表型。此外，一些酶的存在可能在工业过程中作为生物催化剂具有潜在用途。对内生细菌进行基因组测序和分析，并结合比较基因组学，研究其在不同土壤条件下促进植物生长的潜在活性，将加速生物刺激剂在可持续农业中的知识积累和应用。
