State Key Laboratory of Genetic Engineering, Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai 200433, People's Republic of China.
Sci Rep. 2017 Jan 4;7:39819. doi: 10.1038/srep39819.
The C-repeat binding factor (CBF) is crucial for regulation of cold response in higher plants. In Arabidopsis, the mechanism of CBF3-caused growth retardation is still unclear. Our present work shows that CBF3 shares the similar repression of bioactive gibberellin (GA) as well as upregulation of DELLA proteins with CBF1 and -2. Genetic analysis reveals that DELLAs play an essential role in growth reduction mediated by CBF1, -2, -3 genes. The in vivo and in vitro evidences demonstrate that GA2-oxidase 7 gene is a novel CBF3 regulon. Meanwhile, DELLAs contribute to cold induction of CBF1, -2, -3 genes through interaction with jasmonate (JA) signaling. We conclude that CBF3 promotes DELLAs accumulation through repressing GA biosynthesis and DELLAs positively regulate CBF3 involving JA signaling. CBFs and DELLAs collaborate to retard plant growth in response to low temperature.
C 重复结合因子（CBF）对于高等植物的冷响应调控至关重要。在拟南芥中，CBF3 引起生长迟缓的机制尚不清楚。我们目前的工作表明，CBF3 与 CBF1 和 -2 一样，具有抑制生物活性赤霉素（GA）和上调 DELLA 蛋白的相似作用。遗传分析表明，DELLAs 在 CBF1、-2、-3 基因介导的生长抑制中发挥重要作用。体内和体外证据表明，GA2-氧化酶 7 基因是 CBF3 的一个新的调控因子。同时，DELLAs 通过与茉莉酸（JA）信号转导相互作用，有助于 CBF1、-2、-3 基因的冷诱导。我们得出结论，CBF3 通过抑制 GA 生物合成促进 DELLAs 积累，而 DELLAs 通过 JA 信号正向调节 CBF3。CBF 和 DELLA 协同作用以响应低温抑制植物生长。
