Key Laboratory of Regional Environment and Eco-Remediation of Ministry of Educatione, College of Environment, Shenyang University, Shenyang 110044, China.
Sci Rep. 2017 Jan 4;7:39878. doi: 10.1038/srep39878.
Phytoremediation is an effective method to remediate Pb-contaminated soils and root exudates play an important role in this process. Based on gas chromatography-mass spectrometry (GC-MS) and metabolomics method, this study focuses on the comparative metabolic profiling analysis of root exudates from the Pb-accumulating and non-accumulating ecotypes of Sedum alfredii treated with 0 and 50 μmol/L Pb. The results obtained show that plant type and Pb stress can significantly change the concentrations and species of root exudates, and fifteen compounds were identified and assumed to be potential biomarkers. Leaching experiments showed that l-alanine, l-proline and oxalic acid have a good effect to activate Pb in soil, glyceric acid and 2-hydroxyacetic acid have a general effect to activate Pb in soil. 4-Methylphenol and 2-methoxyphenol might be able to activate Pb in soil, glycerol and diethyleneglycol might be able to stabilize Pb in soil, but these activation effect and stabilization effect were all not obvious.
植物修复是一种修复 Pb 污染土壤的有效方法，根系分泌物在此过程中发挥着重要作用。本研究基于气相色谱-质谱联用（GC-MS）和代谢组学方法，聚焦于 Pb 积累型和非积累型高山景天在 0 和 50 μmol/L Pb 处理下的根系分泌物的比较代谢轮廓分析。结果表明，植物类型和 Pb 胁迫可显著改变根系分泌物的浓度和种类，鉴定并假定了十五种化合物为潜在的生物标志物。淋溶实验表明，l-丙氨酸、l-脯氨酸和草酸对激活土壤中的 Pb 具有较好的效果，甘油酸和 2-羟基乙酸对激活土壤中的 Pb 具有一般的效果。4-甲基苯酚和 2-甲氧基苯酚可能能够激活土壤中的 Pb，甘油和二乙二醇可能能够稳定土壤中的 Pb，但这些激活作用和稳定作用均不明显。
