Luo Qing, Sun Lina, Hu Xiaomin, Zhou Ruiren
School of Resources and Civil Engineering, Northeast University, Shenyang, China; Key Laboratory of Regional Environment and Eco-Remediation (Ministry of Education), Shenyang University, Shenyang, China.
Key Laboratory of Regional Environment and Eco-Remediation (Ministry of Education), Shenyang University, Shenyang, China.
PLoS One. 2014 Dec 29;9(12):e115581. doi: 10.1371/journal.pone.0115581. eCollection 2014.
Hydroponic experiments were conducted to investigate the variation of root exudates from the hyperaccumulator Sedum alfredii under the stress of cadmium (Cd). S. alfredii was cultured for 4 days in the nutrient solution spiked with CdCl2 at concentrations of 0, 5, 10, 40, and 400 µM Cd after the pre-culture. The root exudates were collected and analyzed by GC-MS, and 62 compounds were identified. Of these compounds, the orthogonal partial least-squares discrimination analysis (OPLS-DA) showed that there were a distinct difference among the root exudates with different Cd treatments and 20 compounds resulting in this difference were found out. Changing tendencies in the relative content of these 20 compounds under the different Cd treatments were analyzed. These results indicated that trehalose, erythritol, naphthalene, d-pinitol and n-octacosane might be closely related to the Cd stabilization, phosphoric acid, tetradecanoic acid, oxalic acid, threonic acid and glycine could be attributed to the Cd mobilization, and mannitol, oleic acid, 3-hydroxybutanoic acid, fructose, octacosanol and ribitol could copy well with the Cd stress.
进行水培实验以研究镉（Cd）胁迫下超积累植物东南景天根系分泌物的变化。预培养后，将东南景天在添加了浓度分别为0、5、10、40和400 μM CdCl₂的营养液中培养4天。收集根系分泌物并用气相色谱 - 质谱联用仪（GC-MS）进行分析，共鉴定出62种化合物。在这些化合物中，正交偏最小二乘法判别分析（OPLS-DA）表明，不同镉处理的根系分泌物之间存在明显差异，并找出了导致这种差异的20种化合物。分析了这20种化合物在不同镉处理下相对含量的变化趋势。这些结果表明，海藻糖、赤藓糖醇、萘、d-松醇和正二十八烷可能与镉的固定密切相关，磷酸、十四烷酸、草酸、苏糖酸和甘氨酸可能归因于镉的活化，而甘露醇、油酸、3-羟基丁酸、果糖、二十八醇和核糖醇可以很好地应对镉胁迫。
