Butboonchoo Preeyaporn, Wongsawad Chalobol
Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand; The Applied Technology for Biodiversity Research Unit, Science and Technology Research Institute, Chiang Mai University, Chiang Mai 50200, Thailand.
Saudi J Biol Sci. 2017 Jan;24(1):30-35. doi: 10.1016/j.sjbs.2015.09.002. Epub 2015 Sep 9.
The present study determined the prevalence and distribution of gastrointestinal helminths in domestic chickens () between November 2012 and August 2013. One hundred and twenty domestic chickens were purchased from villages in four districts of Phayao province; Mae Chai, Dok Khamtai, Chun and Chiang Kham. Morphological differences were used to identify the helminth species, and HAT-RAPD technique was used to differentiate among closely related species. The results revealed that the total prevalence of infection was 99.2%. Cestode and nematode infections showed the highest prevalence in rainy season, while trematode infections were low and only found in hot season. The species and their prevalence were: (50.8%), (86.7%), (1.7%), (0.8%), (48.3%), (57.5%), (12.5%), sp (35.8%), (14.2%) and sp. (32.5%). The prevalence of helminth infections did not differ significantly between male and female chickens. HAT-RAPD analysis, the specific fragment of 400 and 250 bp indicated that sp. and sp. found, respectively, differ from other closely related species. This study has confirmed that HAT-RAPD technique can be used to differentiate among related species combined with morphological observations.
本研究确定了2012年11月至2013年8月期间家鸡胃肠道蠕虫的流行情况和分布。从帕尧府四个区的村庄购买了120只家鸡；分别来自湄寨、多坎泰、春和清坎。利用形态学差异鉴定蠕虫种类，并使用HAT-RAPD技术区分亲缘关系密切的物种。结果显示，总感染率为99.2%。绦虫和线虫感染在雨季的患病率最高，而吸虫感染率较低，仅在炎热季节发现。其种类及其患病率分别为：（50.8%）、（86.7%）、（1.7%）、（0.8%）、（48.3%）、（57.5%）、（12.5%）、sp（35.8%）、（14.2%）和sp（32.5%）。家鸡的蠕虫感染率在雄性和雌性之间没有显著差异。HAT-RAPD分析表明，分别发现的400和250 bp的特异性片段表明sp和sp与其他亲缘关系密切的物种不同。本研究证实，HAT-RAPD技术结合形态学观察可用于区分相关物种。
