Kumar Brajesh, Smita Kumari, Cumbal Luis, Debut Alexis
Centro de Nanociencia y Nanotecnologia, Universidad de las Fuerzas Armadas ESPE, Av. Gral. Rumiñahui s/n, Sangolqui, P.O. BOX 171-5-231B, Ecuador.
Saudi J Biol Sci. 2017 Jan;24(1):45-50. doi: 10.1016/j.sjbs.2015.09.006. Epub 2015 Sep 6.
Green synthesis of nanoparticles using various plant materials opens a new scope for the phytochemist and discourages the use of toxic chemicals. In this article, we report an eco-friendly and low-cost method for the synthesis of silver nanoparticles (AgNPs) using Andean blackberry fruit extracts as both a reducing and capping agent. The green synthesized AgNPs were characterized by various analytical instruments like UV-visible, transmission electron microscopy (TEM), dynamic light scattering (DLS), X-ray diffraction (XRD) and Fourier transform infrared (FTIR) spectroscopy. The formation of AgNPs was analyzed by UV-vis spectroscopy at = 435 nm. TEM analysis of AgNPs showed the formation of a crystalline, spherical shape and 12-50 nm size, whereas XRD peaks at 38.04°, 44.06°, 64.34° and 77.17° confirmed the crystalline nature of AgNPs. FTIR analysis was done to identify the functional groups responsible for the synthesis of the AgNPs. Furthermore, it was found that the AgNPs showed good antioxidant efficacy (>78%, 0.1 mM) against 1,1-diphenyl-2-picrylhydrazyl. The process of synthesis is environmentally compatible and the synthesized AgNPs could be a promising candidate for many biomedical applications.
使用各种植物材料进行纳米粒子的绿色合成，为植物化学家开辟了新的领域，并减少了有毒化学物质的使用。在本文中，我们报道了一种环保且低成本的方法，该方法使用安第斯黑莓果实提取物作为还原剂和封端剂来合成银纳米粒子（AgNPs）。通过紫外可见光谱、透射电子显微镜（TEM）、动态光散射（DLS）、X射线衍射（XRD）和傅里叶变换红外（FTIR）光谱等各种分析仪器对绿色合成的AgNPs进行了表征。通过紫外可见光谱在435nm波长处分析AgNPs的形成。AgNPs的TEM分析表明形成了晶体、球形且尺寸为12 - 50nm，而在38.04°、44.06°、64.34°和77.17°处的XRD峰证实了AgNPs的晶体性质。进行FTIR分析以鉴定负责AgNPs合成的官能团。此外，发现AgNPs对1,1 - 二苯基 - 2 - 苦基肼显示出良好的抗氧化功效（>78%，0.1mM）。合成过程与环境兼容，并且合成的AgNPs可能是许多生物医学应用的有前途的候选者。
