从水生环境分离出的物种的抗生素抗性分析与表型分析
Antibiotic resistance profiling and phenotyping of species isolated from aquatic sources.
作者信息
Odeyemi Olumide A, Ahmad Asmat
机构信息
Ecology and Biodiversity, Institute of Marine and Antarctic Studies (IMAS), University of Tasmania, Launceston, Australia; School of Biosciences and Biotechnology, National University of Malaysia, Malaysia.
School of Biosciences and Biotechnology, National University of Malaysia, Malaysia.
出版信息
Saudi J Biol Sci. 2017 Jan;24(1):65-70. doi: 10.1016/j.sjbs.2015.09.016. Epub 2015 Sep 14.
This study aimed to investigate antibiotics resistance pattern and phenotyping of species isolated from different aquatic sources in Melaka, Malaysia. A total of 53 species were isolated from the following sources: sediment ( = 13), bivalve ( = 10), sea cucumber ( = 16) and sea water ( = 14) and resistance to 12 antibiotics - Tetracycline (30 μg), Kanamycin (30 μg), Oxytetracycline (30 μg), Ampicillin (10 μg), Streptomycin (10 μg), Gentamicin (10 μg), Sulphamethoxazole (25 μg), Nalixidic acid (30 μg), Trimethoprim (1.25 μg), Novobiocin (5 μg), Penicilin (10 μg) and Chloramphenicol (10 μg) was tested. The results obtained from this study reveal multi drug resistance pattern among the isolates. All the isolates were completely resistant to Ampicillin, Novobiocin, Sulphamethoxazole and Trimethoprim, respectively but susceptible to Tetracycline (100%), Kanamycin (5.7%), Gentamicin (5.7%) and Oxytetracycline (24.5%). Antibiotics phenotyping of the bacteria revealed 21 different phenotypes among the isolates.
本研究旨在调查从马来西亚马六甲不同水源分离出的物种的抗生素耐药模式及表型。总共从以下来源分离出53个物种：沉积物（n = 13）、双壳贝类（n = 10）、海参（n = 16）和海水（n = 14），并测试了它们对12种抗生素的耐药性，这些抗生素分别为：四环素（30μg）、卡那霉素（30μg）、土霉素（30μg）、氨苄青霉素（10μg）、链霉素（10μg）、庆大霉素（10μg）、磺胺甲恶唑（25μg）、萘啶酸（30μg）、甲氧苄啶（1.25μg）、新生霉素（5μg）、青霉素（10μg）和氯霉素（10μg）。本研究获得的结果揭示了分离株中的多重耐药模式。所有分离株分别对氨苄青霉素、新生霉素、磺胺甲恶唑和甲氧苄啶完全耐药，但对四环素（100%）、卡那霉素（5.7%）、庆大霉素（5.7%）和土霉素（24.5%）敏感。对细菌的抗生素表型分析显示，分离株中有21种不同的表型。
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