从感染运动性气单胞菌败血症和爱德华氏菌病的红罗非鱼（罗非鱼属）中分离出的[具体名称未给出]的抗生素和重金属抗性
Antibiotic and heavy metal resistance of and isolated from red hybrid tilapia ( spp.) coinfected with motile aeromonas septicemia and edwardsiellosis.
作者信息
Lee S W, Wendy W
机构信息
Faculty of Agro Based Industry, Universiti Malaysia Kelantan Jeli Campus, 17600, Jeli, Kelantan, Malaysia.
Center for Fundamental and Liberal Education, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia.
出版信息
Vet World. 2017 Jul;10(7):803-807. doi: 10.14202/vetworld.2017.803-807. Epub 2017 Jul 21.
AIM
The aim of this study is to identify antibiogram and heavy metal resistance pattern of and isolated from red hybrid tilapia ( spp.) coinfected with motile aeromonas septicemia and edwardsiellosis in four commercial fish farms.
MATERIALS AND METHODS
and were isolated using glutamate starch phenol red and xylose lysine deoxycholate (Merck, Germany) as a selective medium, respectively. All the suspected bacterial colonies were identified using conventional biochemical tests and commercial identification kit (BBL Crystal, USA). Susceptibility testing of present bacterial isolates to 16 types of antibiotics (nalidixic acid, oxolinic acid, compound sulfonamides, doxycycline, tetracycline, novobiocin, chloramphenicol, kanamycin, sulfamethoxazole, flumequine, erythromycin, ampicillin, spiramycin, oxytetracycline, amoxicillin, and fosfomycin) and four types of heavy metals (mercury, chromium, copper, and zinc) were carried out using disk diffusion and two-fold agar dilution method, respectively.
RESULTS
Three hundred isolates of and were successfully identified by biochemical tests. Antibiotic susceptibility testing results showed that 42.2% of the bacterial isolates were sensitive to compound sulfonamides, sulfamethoxazole, flumequine, oxytetracycline, doxycycline, and oxolinic acid. On the other hand, 41.6% of these isolates were resistant to novobiocin, ampicillin, spiramycin, and chloramphenicol, which resulted for multiple antibiotic resistance index values 0.416. Among tested heavy metals, bacterial isolates exhibited resistant pattern of Zn > Cr > Cu > Hg.
CONCLUSION
Results from this study indicated that and isolated from coinfected farmed red hybrid tilapia were multi-resistant to antibiotics and heavy metals. These resistant profiles could be useful information to fish farmers to avoid unnecessary use of antimicrobial products in the health management of farmed red hybrid tilapia.
目的
本研究旨在确定从四个商业养鱼场中感染运动性气单胞菌败血症和爱德华氏菌病的红色杂交罗非鱼（尼罗罗非鱼属）中分离出的[细菌名称未给出]和[细菌名称未给出]的抗菌谱和重金属抗性模式。
材料与方法
分别使用谷氨酸淀粉酚红和木糖赖氨酸脱氧胆酸盐（德国默克公司）作为选择性培养基分离[细菌名称未给出]和[细菌名称未给出]。所有疑似细菌菌落均通过常规生化试验和商业鉴定试剂盒（美国BBL Crystal）进行鉴定。使用纸片扩散法和两倍琼脂稀释法分别对当前分离出的细菌对16种抗生素（萘啶酸、恶喹酸、复方磺胺甲恶唑、强力霉素、四环素、新生霉素、氯霉素、卡那霉素、磺胺甲恶唑、氟甲喹、红霉素、氨苄西林、螺旋霉素、土霉素、阿莫西林和磷霉素）和四种重金属（汞、铬、铜和锌）进行药敏试验。
结果
通过生化试验成功鉴定出300株[细菌名称未给出]和[细菌名称未给出]。抗生素药敏试验结果表明，42.2%的细菌分离株对复方磺胺甲恶唑、磺胺甲恶唑、氟甲喹、土霉素、强力霉素和恶喹酸敏感。另一方面，41.6%的这些分离株对新生霉素、氨苄西林、螺旋霉素和氯霉素耐药，多重抗生素耐药指数值为0.416。在测试的重金属中，细菌分离株呈现出锌>铬>铜>汞的抗性模式。
结论
本研究结果表明，从同时感染的养殖红色杂交罗非鱼中分离出的[细菌名称未给出]和[细菌名称未给出]对抗生素和重金属具有多重耐药性。这些耐药谱可为养鱼户在养殖红色杂交罗非鱼的健康管理中避免不必要地使用抗菌产品提供有用信息。
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