Mountain Research Centre (CIMO), ESA, Polytechnic Institute of Bragança, Portugal.
Post-Graduation Program of Food Technology (PPGTA), Federal University of Technology - Paraná - UTFPR, Campus Campo Mourão, Brasil.
Food Funct. 2017 Jan 25;8(1):103-110. doi: 10.1039/c6fo01600f.
In the present work, Agaricus bisporus extracts obtained by ultrasound-assisted extraction (UAE), and ergosterol, were incorporated into dairy beverages at concentrations mimicking commercial phytosterol-added yogurts, to work as alternatives. The samples were analysed for nutritional and bioactive properties, and compared with controls (yogurts with no additives or phytosterols), at two storage times (right after incorporation (ST = 0) and after seven days at 4 °C (ST = 7)). The ones incorporated with the extract (YAb) and with ergosterol at the same concentration as in the extract (YPEAb) showed similar antioxidant properties as the ones with phytosterols (YPhy), but a higher cytotoxicity against tumor cells. YPEPhy, the sample with ergosterol at the same amount as phytosterols in YPhy, was the strongest in both bioactivities. For YAb, YPEAb and YPEPhy the antioxidant capacity increased from ST = 0 to ST = 7, meaning that the extract and ergosterol protected the yogurt from oxidation, improving the shelf life. Nutritional parameters were identical for all samples.
在本工作中，采用超声辅助提取（UAE）得到的双孢蘑菇提取物和麦角固醇被掺入到乳制品饮料中，浓度模拟商业添加植物固醇的酸奶，作为替代品。对样品进行营养和生物活性分析，并与对照品（无添加剂或植物固醇的酸奶）进行比较，在两个储存时间（立即掺入时（ST=0）和在 4°C 下储存 7 天后（ST=7））。与对照品相比，添加提取物（YAb）和添加与提取物中相同浓度麦角固醇（YPEAb）的样品显示出与添加植物固醇（YPhy）相似的抗氧化性能，但对肿瘤细胞的细胞毒性更高。YPEPhy 是 YPhy 中植物固醇含量相同的麦角固醇样品，在这两种生物活性中均最强。对于 YAb、YPEAb 和 YPEPhy，抗氧化能力从 ST=0 增加到 ST=7，这意味着提取物和麦角固醇保护酸奶免受氧化，延长了保质期。所有样品的营养参数均相同。
