斑点酶联免疫吸附测定法：一种研究针对乙型肝炎表面抗原免疫反应的敏感体外方法。
The spot-ELISA: a sensitive in vitro method to study the immune response to hepatitis B surface antigen.
作者信息
Wismans P J, van Hattum J, De Gast G C, Endeman H J, Poel J, Stolk B, Maikoe T, Mudde G C
机构信息
Department of Gastroenterology, University Hospital, Utrecht, Netherlands.
出版信息
Clin Exp Immunol. 1989 Oct;78(1):75-9.
Abstract
We present our experience with a new sensitive in vitro method for the study of the immune response to hepatitis B surface antigen (HBsAg). This immunoenzymatic technique, called spot-ELISA, detects specific immunoglobulin production (e.g. IgG- or IgM-anti-HBs) by stimulated individual B cells in vitro. We have studied the immune response against HBsAg by mitogenic stimulation and subsequent spot-ELISA assay in 24 well-documented subjects with known immune status after either natural infection or vaccination with plasma-derived vaccine. Results indicate a good correlation between in vitro IgG anti-HBs spots and immune 'memory.' A possible predictive value in non-immunized and non-responder subjects awaits further confirmation.
摘要
我们介绍了一种用于研究对乙型肝炎表面抗原（HBsAg）免疫反应的新型敏感体外方法的经验。这种免疫酶技术称为斑点酶联免疫吸附测定（spot-ELISA），可在体外检测受刺激的单个B细胞产生的特异性免疫球蛋白（例如IgG或IgM抗HBs）。我们通过有丝分裂原刺激和随后的斑点酶联免疫吸附测定，研究了24名在自然感染或接种血浆源性疫苗后免疫状态已知的记录良好的受试者对HBsAg的免疫反应。结果表明，体外IgG抗HBs斑点与免疫“记忆”之间具有良好的相关性。在未免疫和无反应受试者中的潜在预测价值有待进一步证实。
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