Kovalenko Elena I, Streltsova Maria A, Kanevskiy Leonid M, Erokhina Sophia A, Telford William G
Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry RAS, Moscow, Russian Federation.
National Cancer Institute, National Institutes of Health, Bethesda, Maryland.
Curr Protoc Cytom. 2017 Jan 5;79:9.50.1-9.50.11. doi: 10.1002/cpcy.13.
Our understanding of NK biology is increased dramatically, a product of improved flow-cytometric techniques for analyzing these cells. NK cells undergo significant changes in repertoire during differentiation. A repeating stimulus, such as a cytomegalovirus infection, may result in accumulation of certain types of highly differentiated NK cells designated as memory-like, or adaptive NK cells. Adaptive NK cells are capable of rapid expansion and effective response to the recall stimulus. These cells differ significantly from conventional NK cells both functionally and phenotypically. Here we describe an approach for identification and analysis of adaptive NK cells in human peripheral blood. CD57-positive cells with high expression of activating-receptor NKG2C, increased expression of KIR receptors, lack of co-expression with inhibitory receptor NKG2A, and decreased expression of activating receptor NCR3 (NKp30) all characterize this cell type. The flow-cytometric method described below can identify this NK cell subset on a relatively simple flow cytometer. © 2017 by John Wiley & Sons, Inc.
我们对自然杀伤细胞（NK）生物学的理解有了显著提高，这得益于用于分析这些细胞的流式细胞术技术的改进。NK细胞在分化过程中其库会发生显著变化。反复的刺激，如巨细胞病毒感染，可能导致某些高度分化的NK细胞积累，这些细胞被称为记忆样或适应性NK细胞。适应性NK细胞能够快速扩增并对再次刺激产生有效反应。这些细胞在功能和表型上与传统NK细胞有显著差异。在此，我们描述一种用于鉴定和分析人外周血中适应性NK细胞的方法。具有激活受体NKG2C高表达、KIR受体表达增加、缺乏与抑制性受体NKG2A共表达以及激活受体NCR3（NKp30）表达降低的CD57阳性细胞均为这种细胞类型的特征。以下所述的流式细胞术方法可在相对简单的流式细胞仪上鉴定该NK细胞亚群。© 2017约翰威立国际出版公司
