1 UMR "Emergence des Pathologies Virales" (EPV: Aix-Marseille Univ. - IRD 190 - Inserm 1207 - EHESP), Fondation IHU Méditerranée Infection, APHM Public Hospitals of Marseille , Marseille, France .
2 Centre IRD, UMR MIVEGEC (IRD 224 - CNRS 5290 - Université Montpellier) , Montpellier, France .
Vector Borne Zoonotic Dis. 2017 Jan;17(1):73-80. doi: 10.1089/vbz.2016.1957.
The purpose of this review is to provide practical information to help researchers intending to perform "from field to laboratory" studies on phleboviruses transmitted by sandflies. This guideline addresses the different steps to be considered starting from the field collection of sandflies to the laboratory techniques aiming at the detection, isolation, and characterization of sandfly-borne phleboviruses. In this guideline article, we address the impact of various types of data for an optimal organization of the field work intending to collect wildlife sandflies for subsequent virology studies. Analysis of different data sets should result in the geographic positioning of the trapping stations. The overall planning, the equipment and tools needed, the manpower to be deployed, and the logistics to be anticipated and set up should be organized according to the objectives of the field study for optimal efficiency.
本综述的目的是提供实用信息，帮助有志于开展“从野外到实验室”沙蝇传播黄病毒研究的研究人员。本指南涉及从野外采集沙蝇到旨在检测、分离和鉴定沙蝇传播黄病毒的实验室技术的不同步骤。在本文指南中，我们讨论了各种类型的数据对优化野外工作组织的影响，旨在收集野生动物沙蝇进行后续病毒学研究。对不同数据集的分析应导致诱捕站的地理定位。根据野外研究的目标，应组织总体规划、所需设备和工具、部署的人力以及预期和建立的后勤工作，以实现最佳效率。
