苦碟子注射液减轻大鼠急性脑缺血所致的心肌损伤。
Kudiezi injection mitigates myocardial injury induced by acute cerebral ischemia in rats.
作者信息
Liu Xuemei, Tao Ye, Wang Fengli, Yao Ting, Fu Chen, Zheng Hong, Yan Yan, Liang Xiao, Jiang Xiangning, Zhang Yunling
机构信息
Central Laboratory, Dongfang Hospital, Beijing University of Chinese Medicine, 6 Zone One of Fangxingyuan, Fang Zhuang, Fengtai District, Beijing, 100078, China.
Patent Examination Coperation Center of the Patent Office, SIPO, Beijing, 100190, China.
出版信息
BMC Complement Altern Med. 2017 Jan 5;17(1):8. doi: 10.1186/s12906-016-1514-1.
BACKGROUND
Kudiezi (KDZ) injection is commonly used in traditional Chinese medicine as treatment for cerebral infarction and angina pectoris. The present study investigated the therapeutic effects of KDZ injection on myocardial injury induced by acute cerebral ischemia and the possibly protective mechanisms.
METHODS
Rats were divided into three groups: sham, 6h-ischemia, and KDZ treatment (KDZ). The neurological deficits were determined by the Garcia score. The cerebral infarct volume was measured by 2,3,5-triphenyltetrazolium chloride (TTC) staining, and brain water content was also evaluated. Serum creatinine kinase (CK), lactate dehydrogenase (LDH), and creatine kinase-myocardial band (CK-MB) activity, myocardial tissue malondialdehyde (MDA) levels, L-Glutathione (GSH) levels, and superoxide dismutase (SOD) activity as well as mitochondrial cytochrome c oxidase (COX) activity were determined. Mitochondrial COX I and COX III mRNA expressions of myocardial tissues were measured by RT-PCR.
RESULTS
Impaired neurological function and brain edema were observed in the 6h-ischemia group. TTC staining showed that the 6h-ischemia group had larger infarct zones than the sham group. Myocardial ischemic changes (widened myocardial cell gap, cracks, and obvious edema) were detected in the 6h-ischemia group compared with the sham group, with elevated serum CK-MB activity and CK and LDH levels. Electrocardiography showed lower medium frequency (MF) and high frequency (HF) in the 6h-ischemia group compared with the sham group. In myocardial tissue, COX activity was elevated in the 6h-ischemia compared with the sham group, while SOD, GSH, and MDA levels, and COX I and COX III mRNA expressions remained unchanged. KDZ injection decreased neurological impairment, brain edema, gaps between cells, and infarct size. Compared with the 6h-ischemia group, it reduced serum CK-MB activity and CK and LDH levels, and MDA levels in myocardial tissue. KDZ significantly increased GSH levels, SOD activity, and mitochondria COX activity and the expression of COX I and COX III mRNA in myocardial tissue compared with the sham group.
CONCLUSION
KDZ injection had a protective effect against cerebral ischemia in rats. KDZ injection could also alleviate myocardial injury after acute cerebral ischemia in rats. The possible mechanisms involve the regulation of the oxidative stress/antioxidant capacity after cerebral ischemia.
背景
苦碟子注射液常用于中医治疗脑梗死和心绞痛。本研究探讨苦碟子注射液对急性脑缺血所致心肌损伤的治疗作用及其可能的保护机制。
方法
将大鼠分为三组：假手术组、缺血6小时组和苦碟子治疗组（KDZ组）。通过Garcia评分确定神经功能缺损。采用2,3,5-三苯基氯化四氮唑（TTC）染色测量脑梗死体积，并评估脑含水量。测定血清肌酸激酶（CK）、乳酸脱氢酶（LDH）和肌酸激酶同工酶（CK-MB）活性、心肌组织丙二醛（MDA）水平、L-谷胱甘肽（GSH）水平、超氧化物歧化酶（SOD）活性以及线粒体细胞色素c氧化酶（COX）活性。采用逆转录聚合酶链反应（RT-PCR）检测心肌组织线粒体COX I和COX III mRNA表达。
结果
缺血6小时组观察到神经功能受损和脑水肿。TTC染色显示，缺血6小时组梗死灶面积大于假手术组。与假手术组相比，缺血6小时组检测到心肌缺血改变（心肌细胞间隙增宽、出现裂缝和明显水肿），血清CK-MB活性以及CK和LDH水平升高。心电图显示，缺血6小时组的中频（MF）和高频（HF）低于假手术组。在心肌组织中，与假手术组相比，缺血6小时组COX活性升高，而SOD、GSH和MDA水平以及COX I和COX III mRNA表达保持不变。苦碟子注射液可减轻神经功能损伤、脑水肿、细胞间隙和梗死面积。与缺血6小时组相比，其降低了血清CK-MB活性以及CK和LDH水平，降低了心肌组织中的MDA水平。与假手术组相比，苦碟子显著提高了GSH水平、SOD活性、线粒体COX活性以及心肌组织中COX I和COX III mRNA的表达。
结论
苦碟子注射液对大鼠脑缺血具有保护作用。苦碟子注射液还可减轻大鼠急性脑缺血后的心肌损伤。可能的机制包括调节脑缺血后的氧化应激/抗氧化能力。
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