Ibrahim Abdelaziz E, Abdel-Daim Mohamed Mohamed
Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt.
Department of Pharmacology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt.
Cell J. 2015 Spring;17(1):137-44. doi: 10.22074/cellj.2015.520. Epub 2015 Apr 8.
Tilmicosin (TIL) is a long-acting macrolide antibiotic used to treat cattle for pathogens that cause bovine respiratory disease. However, overdoses of this medication have been reported to induce cardiac damage. Our experimental objective was to evaluate the protective effects of Spirulina platensis (SP) administration against TIL-induced cardiotoxicity in mice.
Our experimental in vivo animal study used 40 male albino mice that were divided into five groups of eight mice per group. The first group served as a control group and was injected with saline. The second group received SP at dose of 1000 mg/kg body weight for five days. The third group received a single dose of TIL (75 mg/kg, subcutaneously). Groups 4 and 5 were given SP at doses of 500 and 1000 mg/kg body weight for five consecutive days just before administration of TIL at the same dose and regimen used for group 3.
TIL treated animals showed a significant increase in serum cardiac injury biomarkers as well as cardiac lipid peroxidation, however they had evidence of an inhibition in antioxidant biomarkers. SP normalized elevated serum levels of lactate dehydrogenase (LDH), creatine kinase (CK), and CK-MB. Furthermore, SP reduced TIL-induced lipid peroxidation and oxidative stress in a dose-dependent manner.
Administration of SP minimized the toxic effects of TIL by its free radicalscavenging and potent antioxidant activity.
替米考星（TIL）是一种长效大环内酯类抗生素，用于治疗牛的由引起牛呼吸道疾病的病原体感染。然而，据报道过量使用这种药物会导致心脏损伤。我们的实验目的是评估给予钝顶螺旋藻（SP）对TIL诱导的小鼠心脏毒性的保护作用。
我们的体内动物实验研究使用了40只雄性白化小鼠，将其分为五组，每组8只。第一组作为对照组，注射生理盐水。第二组以1000 mg/kg体重的剂量给予SP，持续五天。第三组接受单次剂量的TIL（75 mg/kg，皮下注射）。第4组和第5组在按照第3组相同的剂量和方案给予TIL之前，连续五天分别给予500和1000 mg/kg体重的SP。
接受TIL治疗的动物血清心脏损伤生物标志物以及心脏脂质过氧化显著增加，然而它们有抗氧化生物标志物受到抑制的证据。SP使升高的血清乳酸脱氢酶（LDH）、肌酸激酶（CK）和CK-MB水平恢复正常。此外，SP以剂量依赖的方式降低了TIL诱导的脂质过氧化和氧化应激。
给予SP通过其自由基清除和强大的抗氧化活性将TIL的毒性作用降至最低。
