用混养方式利用乙酸进行异养生长将铵转化为眼点拟微绿球藻并提高油脂产量
Detoxification of ammonium to Nannochloropsis oculata and enhancement of lipid production by mixotrophic growth with acetate.
机构信息
Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, PR China.
Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, PR China.
出版信息
Bioresour Technol. 2017 Mar;227:404-407. doi: 10.1016/j.biortech.2016.12.093. Epub 2016 Dec 24.
In this study, the toxicity of ammonium was removed in the microalga Nannochloropsis oculata by using acetate as a carbon source. Algal biomass and lipid production were significantly enhanced when N. oculata was grew on 0.5-50mM of ammonium and 16-64mM of acetate in mixotrophic conditions. When grown mixotrophically on 1mM of ammonium and 32mM of acetate, the biomass and lipid production reached 543mg/L and 279mg/L respectively, which were 1.5 and 9.4times higher than the levels generated when grown autotrophically on nitrate. This suggests that mixotrophic growth with acetate can be a useful method to enhance microalgal lipid production.
在这项研究中，通过使用乙酸盐作为碳源，从微藻眼点拟微绿球藻中去除了氨毒性。当在混养条件下，眼点拟微绿球藻在 0.5-50mM 的铵和 16-64mM 的乙酸盐中生长时，藻生物质和脂质的生产显著增加。当在混养条件下以 1mM 的铵和 32mM 的乙酸盐生长时，生物质和脂质的产量分别达到 543mg/L 和 279mg/L，分别比在硝酸盐自养条件下产生的水平高 1.5 倍和 9.4 倍。这表明，使用乙酸盐的混养生长可以是一种有效提高微藻脂质生产的方法。
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